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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
PCR Based DNA Profiling Standard: 基于DNA分型标准的PCR
- 库存：
1
- 供应商：
甄准生物
- 规格：
6 vials
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文献和实验see a band with 30 cycles you probably won't after 40; rather take an aliquot from the reaction mix and re-PCR with fresh reagents. See here. "Hot Start" PCR: In certain circumstances one wishes to avoid mixing primers and target DNA at low temperatures
Steps for Standard PCR Reaction Design primers. In general, primers should have the following properties: Length of 18-24 bases 40-60% G/C content Start and end with 1-2 G/C pairs
Nanoliter High-Throughput PCR for DNA and RNA Profiling
the time to answer, labor and reagent expenses and workflow complexity. An example of such a system based on a high-density array of nanoliter PCR assays is described here. Functionally equivalent to a microtiter plate, the nanoplate system makes possible
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