Utrophin (N-terminus)

Incorporation of Unnatural Non--Amino Acids into the N-Terminus of Proteins in a Cell-Free Translation System

, the incorporation of unnatural α-amino acids and non-α-amino acids into the N-terminus of proteins has been achieved using expanded initiation codons. Here, we describe the method for the incorporation of fluorescent-labeled non-α-amino acids into the N-terminus

被拒稿 N 次后，含泪总结这份策略!

研究人员的论文发表之路往往很难一帆风顺，也就是说，通常情况下，很少有论文一经投稿、审稿就直接被录用发表。 一般来说，在审稿后收到「小修（minor revision）」的修改意见，已经可谓是十分令人兴奋、皆大欢喜的情况了，论文已在很大程度上接近了被录用发表。然而，更多时候，稿件在经历同行评审之后，可能会连同大量的修改意见一起被返回给作者修改，也就是「大修（major revision）」，这意味着稿件难免要面临一番大动干戈、脱胎换骨的修改，即便如此，依然不能保证修改后的稿件能被顺利录用发表

Modification of the 5′ Terminus of Oligodeoxyribonucleotides for Conjugation with Ligands

can be introduced at the 5? terminus of an oligonucleotide by adding a linker to the ligand and modifying the 5? terminus of the oligonucleotide. These are then reacted to give the ligand?oligonucleotide conjugate. This unit describes the addition of carboxylated