人外周血 CD4+ cd25- 初始T细胞(进口)；hPB

人外周血 CD4+ cd25- 初始T细胞(进口)；hPB CD4+ CD25- T cells
英文名称：CD4+ CD4+ Naive Human Peripheral Blood T Cells
规格：1×10^6 cells/管（冻存管，1mL/管）；或1×10^6 cells/T25培养瓶（复苏后培养）
产品简介：人外周血 CD4+ cd25- 初始T细胞；hPB CD4+ CD25- T cells属于人源外周血来源的正常细胞。该细胞在体外可稳定增殖，呈悬浮生长，形态为圆形、大小均一，广泛用于细胞生物学、毒理学评价及体外模型构建等研究方向。本品经种属与形态学鉴定，种属来源明确、形态特征典型，可提供稳定可重复的实验结果。
基本信息：细胞名称：人外周血 CD4+ cd25- 初始T细胞；hPB CD4+ CD25- T cells；种属：人；组织来源：外周血；细胞类型：T细胞；生长特性：悬浮；完全培养基：RPMI-1640 + 10% FBS；培养条件：37℃、5% CO2；传代比例：1:2-1:3；冻存条件：90% FBS + 10% DMSO；生物安全等级：BSL-2
产品优势：①种属鉴定合格，种属来源清楚，形态典型；②代次低、活力高，复苏后存活率≥90%，贴壁/增殖状态稳定；③支原体、细菌、真菌检测均为阴性，无外源因子污染；④批次稳定，可长期重复供货，配套完整培养方案；⑤提供鉴定报告与批次质检记录，数据可追溯
产品售后：收到细胞后7天内，如出现污染、贴壁不良、存活率低或鉴定不符等问题，经核实可免费更换或补发；提供全程技术支持（培养基配制、复苏传代、冻存与实验设计建议）；可应要求出具STR/种属鉴定报告、支原体检测报告及批次质检记录。
储存条件：液氮（-196℃）气相长期保存；-80℃冰箱仅可短期存放（不超过3个月）；避免反复冻融，冻存管取出后于37-40℃水浴快速解冻并在2分钟内转移至完全培养基。
运输条件：冻存管采用干冰运输（全程维持-70℃以下）；T25培养瓶采用常温运输（灌满完全培养基、密封防漏），收到后用75%酒精消毒表面，静置培养箱24小时后再观察处理。
物种来源：种属：人（Human）；组织来源：外周血
细胞形态：圆形、大小均一，呈单个或小簇状悬浮生长
生长状态：悬浮生长，细胞成团或单个悬浮于培养基中，每2-3天半量换液
年限：液氮条件下长期保存，有效保存期5年；复苏后建议连续传代不超过10代（原代细胞建议3-5代内使用），代次过高可能引起表型漂移与增殖能力下降。
免疫类型：人源细胞，属异种移植背景；进行体内移植时需使用免疫缺陷小鼠（如BALB/c nude、NOD-SCID、NSG），体外培养不涉及免疫排斥；如用于免疫学实验应关注人源MHC与小鼠免疫系统的匹配性。本细胞本身为免疫细胞，培养与移植时需注意细胞表面免疫标志与受体匹配。
质量控制与检测：种属鉴定无误、形态学特征典型；支原体检测阴性；细菌、真菌无菌检测阴性；细胞活力≥90%；内毒素检测合格
传代方法：无需胰酶消化，直接按1:2-1:3比例吸取细胞悬液接种于新鲜完全培养基；或1000rpm离心5分钟弃上清后重悬接种，每2-3天换液一次
培养条件：37℃、5% CO2、饱和湿度培养箱静置培养；每2-3天换液或传代一次，维持密度5×10^5-2×10^6 cells/mL
培养基：RPMI-1640 + 10% FBS
CD4+ CD4+ Naive Human Peripheral Blood T Cells (imported)
English Name: CD4+ CD4+ Naive Human Peripheral Blood T Cells
Specifications: 1×10^6 cells/vial (cryovial, 1 mL/vial); or 1×10^6 cells/T25 flask (cultured after recovery)
CD4+ CD4+ Naive Human Peripheral Blood T Cells is a normal cell line derived from human peripheral blood. These cells proliferate stably in vitro, showing growth in suspension, a round and uniform-size morphology, and are widely used in research on cell biology, toxicological evaluation, and in vitro model construction. This product has passed species and morphological identification, with a clear species origin and typical morphological features, ensuring stable and reproducible experimental results.
Basic Information: Cell name: CD4+ CD4+ Naive Human Peripheral Blood T Cells (hPB CD4+ CD25- T cells); Species: Human; Tissue source: Peripheral blood; Cell type: T cells; Growth characteristics: Suspension; Complete medium: RPMI-1640 + 10% FBS; Culture conditions: 37°C, 5% CO2; Subculture ratio: 1:2–1:3; Cryopreservation: 90% FBS + 10% DMSO; Biosafety level: BSL-2
Product Advantages: ① Species identification passed, with a clear species origin and typical morphology; ② Low passage number and high viability, with a post-thaw survival rate of ≥90% and stable adherence/proliferation; ③ Negative for mycoplasma, bacteria, and fungi, with no adventitious agent contamination; ④ Stable batches for long-term repeat supply, with a complete culture protocol; ⑤ Identification reports and batch quality inspection records available for traceability
After-Sales Service: Within 7 days of receiving the cells, if issues such as contamination, poor adherence, low viability, or failed authentication occur, free replacement or resupply will be provided after verification. Full technical support is available (medium preparation, recovery and subculture, cryopreservation, and experimental design advice), and STR/species identification reports, mycoplasma testing reports, and batch quality inspection records can be issued upon request.
Storage Conditions: Long-term storage in the gas phase of liquid nitrogen (-196°C); the -80°C freezer is suitable only for short-term storage (no more than 3 months). Avoid repeated freeze-thaw cycles; after removing the cryovial, thaw it rapidly in a 37-40°C water bath and transfer it to complete medium within 2 minutes.
Shipping Conditions: Cryovials are shipped on dry ice (maintained below -70°C throughout); T25 flasks are shipped at ambient temperature (filled with complete medium and sealed against leakage). Upon receipt, disinfect the surface with 75% ethanol and leave the cells in the incubator for 24 hours before observation and handling.
Species and Source: Species: Human; Tissue source: Peripheral blood
Cell Morphology: Round and uniform in size; suspension growth as single cells or small clusters
Growth Status: Suspension growth; cells grow singly or in clusters in the medium; half-medium renewal every 2-3 days
Storage Life and Passage Limit: Long-term preservation under liquid nitrogen with a valid storage period of 5 years; after recovery, continuous passaging of no more than 10 passages is recommended (primary cells are recommended for use within 3-5 passages), as an excessively high passage number may cause phenotypic drift and reduced proliferative capacity.
Immunological Background: Human cells, with a xenotransplantation background; in vivo transplantation requires immunodeficient mice (e.g., BALB/c nude, NOD-SCID, NSG), while in vitro culture does not involve immune rejection; for immunological experiments, the compatibility between human MHC and the murine immune system should be considered.These cells are themselves immune cells; during culture and transplantation, attention should be paid to the matching of cell-surface immune markers with the corresponding receptors.
Quality Control and Testing: Species identification confirmed, with typical morphological features; mycoplasma testing negative; sterility testing negative for bacteria and fungi; cell viability ≥90%; endotoxin testing passed
Subculture Method: No trypsin digestion is required; directly transfer the cell suspension into fresh complete medium at a ratio of 1:2-1:3, or centrifuge at 1000 rpm for 5 minutes, discard the supernatant, resuspend, and inoculate; renew the medium every 2-3 days
Culture Conditions: Static culture in a 37°C, 5% CO2, saturated-humidity incubator; renew the medium or subculture every 2-3 days, maintaining a density of 5×10^5-2×10^6 cells/mL
Culture Medium: RPMI-1640 + 10% FBS

FAQ（选取前2个问题）	问：多久可以传代	答：一般情况下70%–80%可以传代
	问：如何发货	答：复苏好的T25瓶发货 ，冻存的干冰发货