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文献和实验Streptomyces Phospholipase D Cloning and Production
The transphosphatidylation catalytic ability of phospholipase D (PLD, EC 3.1.4.4) is a powerful biochemical tool for the acquisition of rare phospholipids (PLs), e.g., phosphatidylglycerol (PG) and phosphatidylserine (PS), and artificial
Measurement of Phospholipase D Activation in Vascular Smooth Muscle Cells
Phospholipase D (PLD), which hydrolyzes phospholipids (primarily phos-phatidylcholine) to generate phosphatidic acid, is an essential component in cellular signal transduction (1 ,2 ). Phosphatidic acid and its dephosphorylated product
Measuring PLD Activity In Vivo
of a unique property of PLD, i.e., its ability to substitute a primary alcohol, such as 1-butanol, for water in the hydrolytic reaction. This transphosphatidylation reaction results in the formation of phosphatidylbutanol (PBut), which is a specific and unique
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