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- 文献和实验
- 技术资料
- 英文名:
RNase H
- 供应商:
合肥瑞辕生物科技有限公司
- 规格:
250U
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文献和实验RNase H: Specificity, Mechanisms of Action, and Antiviral Target
The Ribonuclease (RNase) H is one of the four enzymes encoded by all retroviruses, including HIV. Its main activity is the hydrolysis of the RNA moiety in RNA–DNA hybrids. The RNase H ribonuclease is essential in the retroviral life cycle
Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold
scaffold, using RNase H and two DNA oligonucleotides. After cleavage, we show that the RNA of interest can be isolated in a one-step purification. This method has, in particular, applications in structural investigations of RNA.
Oligonucleotide-Targeted RNase H Protection Analysis of RNA-Protein Complexes
This chapter focuses on the analysis of RNA-protein complexes (RNPs, ribonucleoprotein particles) by oligonucleotide-targeted RNase H digestion, a powerful approach to probe the domain structure of an RNP, both in crude extracts
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