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- 文献和实验
- 技术资料
- 英文名:
SR RESIN 100-150μm
- 供应商:
合肥瑞辕生物科技有限公司
- 规格:
100g
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文献和实验Coupling Peptides To Resin For Affinity Purification
is light sensitive. Wash with 10 volumes of 0.1 M NaPi pH 7.8= buffer. Resuspend the resin as a 50% slurry in buffer. Add the peptide. If the peptide is soluble directly in buffer, add it as a solid. Many peptides go in better added as a 100
Coupling Peptides To Resin For Affinity Purification
is the same. Some hydrophobic peptides will crash out. It is possible to couple such peptides in 20% buffer, 80% DMSO. In an extreme case you can use 100% DMSO containing triethylamine. The amount of peptide to add is a question. We usually add 1-2 mg peptide/ml resin, hoping
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
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