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- 文献和实验
- 技术资料
- 供应商:
合肥瑞辕生物科技有限公司
- 保修期:
Nylon Membrane Filters, 5.0 Micron, 200 x 200mm, 5/Pk
- 规格:
P5
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文献和实验Chick Chorioallantoic Membrane (CAM) Assay
Dickinson, Bedford, MA) was diluted to a final concentration of 10 mg/ml with DMEM and directly pipetted onto meshes. Nylon mesh with 250 μm2 openings were cut into 4 mm x 4 mm squares and autoclaved. In preparation for polymerization, meshes were placed
bacteria on a separate plate. Select plates that have an optimal density of bacteria (i.e. 2 mm spacing between colonies). Put selected plates at 4°C for one hour. Lay 83 mm circular nylon membrane on surface of agar plate
Hybridization of High Density Arrayed BAC Nylon Filter Blots
Protocol for hybridization of high density arrayed Bacterial Artificial Chromosome nylon filter blots with 100 PCR isolated Unigene cDNA inserts, pooled in a combined probe Isolation and purification of cDNA inserts using polymerase
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