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- sterlitech
- PET1020030
- 2026年09月27日
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- 文献和实验
- 技术资料
- 供应商：
合肥瑞辕生物科技有限公司
- 保修期：
Polyester (PETE) Membrane Filters, 1.0 Micron, 200 x 250mm, 30/Pk
- 规格：
P30
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文献和实验Overgo Probing of High-Density Filters
1.50 ml of warmed hyb solution is added to a 30 cm x 4 cm hyb bottle. Filters are prewet with warmed 2xSSC and then each filter is rolled and inserted into the bottle. With the cap on, the bottle is rotated to allow the filter to unroll slowly
the desired fraction in 12mls of 140mM NaCl and 10mM Tris. put in SW41 rotor buckets and weigh. Spin in the ultracentrifuge at 4C at ? x g (8000 RPM) for 30min. After spinning, aspirate off the buffer and resuspend the pellet in 500ul of 10
Chick Chorioallantoic Membrane (CAM) Assay
Dickinson, Bedford, MA) was diluted to a final concentration of 10 mg/ml with DMEM and directly pipetted onto meshes. Nylon mesh with 250 μm2 openings were cut into 4 mm x 4 mm squares and autoclaved. In preparation for polymerization, meshes were placed
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