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- 文献和实验
- 技术资料
- 供应商：
合肥瑞辕生物科技有限公司
- 保修期：
Polyester (PETE) Membrane Filters, 8.0 Micron, 25mm, 100/Pk
- 规格：
100/Pk
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文献和实验MitoProbe DiIC1 Mitochondrial Membrane Potential Protocol
, phosphate-buffered saline, or other buffer at approximately 1 x 106 cells/mL. 2) For the control tube, add 1 μL of 50 mM CCCP (supplied with the kit, 50 μM final concentration) and incubate the cells at 37°C for 5 minutes.Note: CCCP
bacteria on a separate plate. Select plates that have an optimal density of bacteria (i.e. 2 mm spacing between colonies). Put selected plates at 4°C for one hour. Lay 83 mm circular nylon membrane on surface of agar plate
with a salt solution (300 mM NaCl) of high ionic strength. (C ) Ca2+ induces defects on a supported membrane containing 25% POPA. Defects appear upon addition of 0.5 mM CaCl2 and gradually increase in size with time. (D ) and (E ) Examples of diffusion
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