CEFT Pool (PM-CEFT-4)

Tubulin Preparat

remove the supernatants from the pellets, which are sometimes loose, and pool in a graduated cylinder. Note the volume (V2). 16. Add an equal volume of PM-8M, mix gently and store at -20°C. The tubulin at this stage

【共享】酵母电转改进PROTOCOL

volume)Day 11.Inoculate 5mls YPD (in a 50 ml tube) with yeast strain (single colony on YPD plate or scraping from glycerol stock). Incubate O/N at 28-30 degrees, shaking.Day 22.In early afternoon (3pm), inoculate 100mls of YPD (500ml flask) with 100ul of O/N

Porcine brain tubulin preparation

Homogenization buffer (freshly prepared) 1L PM buffer 100 mM MgATP 1L PMG Buffer 100 mM MgGTP 1M dithiothreitol (DTT) Glutamic acid sodium salt 实验设备   Waring Blender Temperature controlled ultracentrifuge