MKL-A666752-1mg,Anti-LILRB2 &

Analysis of RNA-Protein Complexes by RNA Coimmunoprecipitation and RT-PCR Analysis from Caenorhabditis elegans

16-27) describes how to immunopurify RNA-protein complexes from worm extract in two steps. First, the extract is incubated with the antibodies to allow binding of the antibody to its target protein. Second, the antibody with the associated protein

RNA-chromatin immunoprecipitations (RNA-ChIP) in mammalian cells

in each immunoprecipitation will vary. Two of the important variables include the characteristics of the protein target and the antibody used. Use of higher-affinity/efficiency antibodies or targeting of histone proteins, for instance, may favor detection of the RNA-protein

Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos

. The strip is baked for 30 min in a vacuum oven at 80oC, washed once in 2 x SSC, twice in PBT, and blocked for 30 minutes in PBT. The strip is then incubated for 30-60 min with AP-anti DIG antibody diluted 1:2000 in PBT. After three 10 min washes in PBT