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- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human HER3/ERBB3 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100.00 µg/1.00 mg
| 规格: | 100.00 µg | 产品价格: | ¥3870.0 |
|---|---|---|---|
| 规格: | 1.00 mg | 产品价格: | ¥21290.0 |
重组人GM-CSF / CSF2蛋白(产品说明)
蛋白名称:重组人GM-CSF / CSF2蛋白
蛋白构建:A DNA sequence encoding human GMCSF (NP_000749.2) (Met1-Glu144) was expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS(Routinely tested).
生物活性:1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.06-0.3 ng/mL. 2.Western blot analysis of extracts from serum-starved TF-1, untreated (-) or treated with GM-CSF (Cat: 10015-HNAH)(100 ng/mL, 5 min; +), using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb(Cat: 111160-R0023) at 1:1000 dilution (upper) or Anti-STAT5a Antibody, Rabbit PAb, Antigen Affinity Purified (Cat: 107353-T32) at 1:500 dilution (lower)(Routinely tested).
预测N端:Ala 18
蛋白分子量:The recombinant human GMCSF consists of 127 amino acids and predicts a molecular mass of 14.5 kDa. As a result of glycosylation, it migrates as an approximately 19-29 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 19.7 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号:NP_000749.2
蛋白氨基酸序列:Met1-Glu144
蛋白名称:重组人GM-CSF / CSF2蛋白
蛋白构建:A DNA sequence encoding human GMCSF (NP_000749.2) (Met1-Glu144) was expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS(Routinely tested).
生物活性:1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.06-0.3 ng/mL. 2.Western blot analysis of extracts from serum-starved TF-1, untreated (-) or treated with GM-CSF (Cat: 10015-HNAH)(100 ng/mL, 5 min; +), using Phospho-Stat5 (Tyr694) Antibody, Rabbit MAb(Cat: 111160-R0023) at 1:1000 dilution (upper) or Anti-STAT5a Antibody, Rabbit PAb, Antigen Affinity Purified (Cat: 107353-T32) at 1:500 dilution (lower)(Routinely tested).
预测N端:Ala 18
蛋白分子量:The recombinant human GMCSF consists of 127 amino acids and predicts a molecular mass of 14.5 kDa. As a result of glycosylation, it migrates as an approximately 19-29 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 19.7 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号:NP_000749.2
蛋白氨基酸序列:Met1-Glu144
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文献和实验相关实验
子量的化合物可以进入孔中,滞留时间长;大分子量的化合物不能进入孔中,直接随流动相流出。它利用分子筛对分子量大小不同的各组分排阻能力的差异而完成分离。常用于分离高分子化合物,如组织提取物、多肽、蛋白质、核酸等。 被分离组分在柱中的洗脱原理 II.基本概念和理论
相是可以溶解样品的溶剂。小分子量的化合物可以进入孔中,滞留时间长;大分子量的化合物不能进入孔中,直接随流动相流出。它利用分子筛对分子量大小不同的各组分排阻能力的差异而完成分离。常用于分离高分子化合物,如组织提取物、多肽、蛋白质、核酸等。 被分离组分在柱中的洗脱原理 II.基本概念和理论 一、基本概念和术语 1.色谱图和峰参数 色谱图(chromatogram)——样品流经色谱柱和检测器,所得到的信号-时间曲线,又称色谱流出曲线(elution profile)。 基线(base
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重组人HER3/ERBB3蛋白 (His 标签), 10201-H08H1
¥3870 - 21290











