M5655-5X1G默克Merck Sigma-Aldric

基于PCR技术的染色质沉淀分析

of H2O:54 g of Tris base27.5 g of boric acid20 ml of 0.5 M EDTA (pH 8.0)The 0.5x working solution is 45 mM Tris-borate/1 mM EDTA.TBE is usually made and stored as a 5x or 10x stock solution. The pH of the concentrated stock buffer should be approx

Neutralizing Bioassay Protocols

with 10% FBS) Assay Medium (RPMI supplemented with 10% FBS) 96-well flat-bottom culture plate (Costar Cat. No. 3595) MTT solution (Sigma Cat. No. M5655) 5mg/ml stock in PBS kept at room temperature (protect from light) MTT

Oligonucleotide Purification

Solutions Gel Stocks Diluent 5X Buffer 25% Acrylamide 209 g Urea 209 g Urea 209 g Urea up to 500 ml Q 250 ml 10X TBE 120.8 g Acrylamide up to 500 ml Q 4.1 g BIS up to 500 ml Q 2.5 M NH4 OAc 19.2 g NH4 OAc up to 100 ml Q Formamide Dye 9 ml