M8410-5ML默克Merck Sigma-Aldrich

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of aggregation plate Materials:       Dissecting microscope;       Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3);       1 ml syringe with 26 G 1/2 needle of KSOM medium;       light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);       

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

References: 1.      Sigma-Aldrich. Target ID Library Technical Bulletin. Catalog # MREH01. www.sigma-aldrich.com. 2.      Huang, Q., Gumireddy, K., Schrier, M., et al. The microRNAs miR-373 and miR-520c promote tumour invasion

Crystallization of Kinesin Family Motor Proteins

MgCl2, 2 mM DTT was pre-incubated with 4 mM AMP*PNP or ATP for 2 hoursCrystals grew in 11% PEG 8000, 0.8 M NaCl, 50 mM Na2H2PO4, pH 6.8, 7 mM DTTYun et al., 2003Rat Kinesin-1 rK354Hanging drop Room temperature9-14 mg/ml protein 20 mM PIPES pH 7.5, 50 mM