M9768-50G默克Merck Sigma-Aldrich

Synthesis of Building Blocks and Oligonucleotides with {G}O6‐Alkyl‐O6{G} Cross‐Links

., Noronha, A.M., Murphy, S.P., Wilds, C.J., Tubbs, J.L., Tainer, J.A., Chowdhury, G., Guengerich, F.P. and Pegg, A.E. 2008. Repair of O6‐G‐alkyl‐O6‐G interstrand cross‐links by human O6‐alkylguanine‐DNA alkyltransferase. Biochemistry 47:10892‐10903

Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins

Ice Charcoal slurry: 5% (w/v) activated charcoal (e.g., Sigma, cat. no. C4386), 50 mM phosphoric acid, pH 3.0; stored stably long‐term at 4°C

Replication timing by density transfer

for ~1 min. You can empirically determine how long it takes to bring your desired volume of medium from 37o to 23o C. 6. Collecting samples: Freeze 8 ml of 0.1% Na-azide, 0.2 M EDTA in "50 ml" Oakridge-type plastic, screw-capped centrifuge tubes