M9768-5G默克Merck Sigma-Aldrich

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

: Adenosine (5a ) or 2′‐deoxyadenosine monohydrate (4a ) (Sigma‐Aldrich) Cytidine (5c ), 2′‐deoxycytidine monohydrochloride (4c ), guanosine (5g ), 2′

Blocking cDNA arrays printed on amine slides

slides in dish Put on stir plate and verify stir bar will not hit the slides. ____3. Weigh out succinic anhydride (Aldrich #23,969-0, Lot # _________) stored under desiccation) 3.5 g for 200 mls. Keep in desiccator. Bring to hood

核酸、蛋白技术常用贮存液的配制

的溶液含有100%（M/V）TCA。 32．1mol/L Tris溶液 【配制方法】 在800ml水中溶解121.91g Tris碱，加入浓HCl调节　pH值至所需值。 pH　HCl 7.4 70ml 7.6 60ml 8.0 42ml 应使溶液冷至室温后方可最后调定pH值，加水定容至1L，分装后高压灭菌。 【注意】 如1mol/L溶液呈现黄色，应予丢弃并置备质量更好的Tris。 尽管多种类型的电极均不能准确测量Tris溶液的pH值，但仍可向大多数厂商购得合适的电极