相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
AJE 润色服务限时 8 折起,权威机构信任 15+ 年Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 库存:
现货
- 现货状态:
现货
- 供应商:
上海然其生物科技有限公司
- 规格:
50个/包/24包/箱
| 规格: | 50个/包 | 产品价格: | ¥269.53 |
|---|---|---|---|
| 规格: | 24包/箱 | 产品价格: | ¥6469.0 |
NUNC 379146 4.5 mL 冻存管(内旋,自立星形防旋转底座)
中文介绍
货号:379146(分装小包别名 379146PK) 品牌:Thermo Scientific™ Nunc™ 名称:Nunc 生物样本库与细胞培养冻存管,4.5mL,内旋盖,锥形自立 Starfoot 星型 A-R 防旋转底座,带白色书写区
基础参数
- 材质:医用原生聚丙烯 PP;内旋盖带硅胶密封垫圈
- 容量:4.5 mL;底部:锥形自立星型防旋转 A-R 底座,台面可直立放置
- 螺纹类型:内旋螺纹,硅胶密封圈实现可靠密封
- 适用温度:+4℃ ~ 液氮气相 - 196℃;不建议直接浸泡在液相液氮中
- 灭菌:γ 辐照灭菌,SAL=10⁻⁶;无 RNase、无 DNase、无热原、无细胞毒性、BSE/TSE 无风险
- 标识:管身大面积白色手写标记区域,便于样本编号溯源
- 尺寸:外径 12.5 mm,管高 92 mm
- 包装规格:300 支 / 小包(379146PK);1200 支 / 整箱
产品特点
- 内旋盖 + 内置硅胶密封圈,低温环境密封性能优异,有效防止液氮渗入、样本泄漏,适合长期超低温归档;内旋设计,降低开盖时手套接触管口污染样本的风险。
- Starfoot 星型 A-R 防旋转自立底座,可直接直立放置于操作台;拧盖时管体不会旋转,单人操作便捷,适配标准冻存盒与自动化样本库设备。
- 医用级原生聚丙烯,低温韧性优异,长期 - 80℃或液氮气相储存不易脆裂,保护珍贵生物样本。
- 洁净车间生产,无核酸酶、无热原,满足基因组、转录组、多组学临床生物样本保存需求,符合生物样本库建库规范。
- 兼容各类自动化样本存储、扫码管理系统。
适用场景
细胞株与原代细胞冻存、临床活检组织 / 血液 / PBMC 样本分装保存、生物样本库长期归档、多组学标志物样本保藏、临床诊断样本低温存储。
区分同系列:379146 内旋、锥形自立星脚;337516 为 4.5mL 外旋自立冻存管,二者螺纹方向不同,不要混淆;363452 为内旋普通圆底(非星脚)版本。
English Introduction
Catalog No.: 379146 (also known as 379146PK) Brand: Thermo Scientific™ Nunc™ Name: Nunc Biobanking and Cell Culture Cryogenic Tube, 4.5 mL, internal thread, self-standing conical starfoot A-R base with writing area
Specifications
- Material: Virgin medical grade Polypropylene (PP); internal cap with silicone gasket
- Volume: 4.5 mL; bottom: Conical self-standing starfoot anti-rotation A-R base
- Thread: Internal thread with silicone sealing gasket
- Temperature range: +4 ℃ to -196 ℃ (vapor phase liquid nitrogen). Not for direct immersion in liquid LN₂.
- Sterilization: Gamma irradiation, SAL=10⁻⁶; RNase-free, DNase-free, non-pyrogenic, non-cytotoxic, BSE/TSE free
- Marking: Large white writing area on tube body
- Dimensions: OD 12.5 mm, height 92 mm
- Packaging: 300 tubes per pack (379146PK); 1200 tubes per case
Key Features
- Internal thread cap with integrated silicone gasket provides reliable sealing under ultra-low temperature, prevents LN₂ ingress and sample leakage for long-term cryoarchiving. Internal thread design reduces contamination risk from glove contact during cap opening.
- Starfoot anti-rotation self-standing A-R base enables upright placement on bench; prevents tube twisting during cap manipulation. Compatible with standard cryoboxes and biobank automation systems.
- Virgin polypropylene maintains high toughness under cryogenic conditions, resistant to cracking at -80 ℃ and LN₂ vapor phase for valuable biosample protection.
- Manufactured in cleanroom, nuclease-free and endotoxin-free, suitable for genomic, transcriptomic and multi-omics clinical biosample banking.
- Compatible with automated biobank storage and barcode tracking systems.
Applications
Cryopreservation of cell lines and primary cells, aliquot & archiving of clinical biopsies, blood and PBMC samples, long-term biorepository storage, multi-omics biomarker sample preservation and diagnostic biosample archiving.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验- Multi-omics profiling of human gastric cancer patient-derived xenograft samples in biobank,Gut, 2022, IF=31.79
Methods:Human gastric PDX tumor tissues and isolated PBMCs were aliquoted into Nunc 4.5 mL internal-thread starfoot self-standing cryotubes, archived at vapor phase liquid nitrogen for subsequent multi-omics sequencing analysis. 用途:胃癌 PDX 肿瘤组织、外周血样本分装,生物样本库液氮长期归档、多组学测序(匹配整套肿瘤实验体系)
- Functional preservation of frozen primary human alveolar macrophages for immunological research,Journal of Immunology, 2022
Methods:Isolated primary human alveolar macrophages were resuspended in cryomedium and transferred to Nunc 4.5 mL internal thread starfoot self-standing cryogenic tubes for -80 ℃ and LN₂ vapor phase cryopreservation. 用途:人原代巨噬细胞冻存,冻存后细胞表型与功能保留研究,免疫学原代细胞样本库
- Biobanking of human plasma samples for cancer biomarker discovery,Clinical Chemistry and Laboratory Medicine, 2023
Methods:Human plasma biosamples were dispensed into Nunc 4.5 mL internal-thread self-standing starfoot cryotubes and stored at -80 ℃ for long-term biomarker research. 用途:人血浆样本库构建,肿瘤生物标志物多组学研究
mm培养皿培养。24h后换成DF12+N2的无血清培养液,此后每三天半量换液1次。 瓶底星形胶质细胞的继续培养 25cm2培养瓶内的星形胶质细胞用D-Hanks液洗2次后常规传代,以1.5×105/cm2种入预先用100μg/L多聚赖氨酸包被好的35mm培养皿培养。培养液为GM,每三天半量换液1次。 动脉粥样硬化患者VSMC的提取 实验材料:眼科剪刀1把 眼科镊子2把 1×PBS约200ml M199培养液 离心机 5ml小皿 10ml大皿 实验步骤: 1.在超净台内用10ml的离心
/ml密度,在包含有血清的冷冻培养基中再次悬浮细胞,或者以0.5×107到1×107在无血清培养基中,再次悬浮细胞。 分装进冻存管,将冻存管置于湿冰上或放入4℃冰箱中,5分钟内开始冷冻步骤。 细胞以1℃/分钟进行冷冻,可以通过可编程序的冷冻器进行或者把隔离盒中的冻存管放到-70℃到-90℃的冰箱中,然后转移到液氮中贮存。 2、贴壁细胞 使用分离试剂从基层分离细胞,分离时尽可能温和,使对细胞的损伤减少到最小。 在完全生长培养基中,再次悬浮分离细胞,确定有活力细胞数。 以大约
技术资料暂无技术资料 索取技术资料










