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- 文献和实验
- 技术资料
- 库存:
现货
- 现货状态:
现货
- 供应商:
上海然其生物科技有限公司
- 规格:
50个/包/24包/箱
| 规格: | 50个/包 | 产品价格: | ¥261.74 |
|---|---|---|---|
| 规格: | 24包/箱 | 产品价格: | ¥6281.75 |
NUNC 337516 4.5mL 可立外旋冻存管
中文介绍
货号:337516(别名 337516PK) 品牌:Thermo Scientific™ Nunc™ 名称:Nunc 生物样本库及细胞培养冻存管,4.5mL,外旋盖,圆形自立可立底,带书写区
基础参数
- 管体材质:医用级聚丙烯 PP;管盖:聚乙烯 PE
- 容量:4.5 mL;底部:圆形自立可立 Starfoot 星型底座,防旋转(A-R Base),台面可直接直立放置
- 螺纹类型:外旋螺纹,降低开盖时手部污染样本风险,更利于无菌操作
- 温度耐受:+4℃ ~ 液氮气相 - 196℃(⚠️不建议直接浸泡在液氮液相)
- 灭菌:γ 辐照灭菌,SAL=10⁻⁶;无 RNase、无 DNase、无热原、无细胞毒性、无 BSE/TSE
- 管身:预留大面积白色书写标记区,可手写样本编号信息
- 包装规格:50 支 / 小包;300 支 / 中包;1200 支 / 整箱
产品特点
- 星型防旋转自立底座,放在台面无需冻存架即可直立放置,样本分装操作便捷;防旋转底座,拧盖时管子不会跟着转动
- 外旋螺纹设计,管体外螺纹,减少开盖过程中手套触碰管口内壁,降低样本交叉污染风险,适合生物样本库、原代细胞样本保存
- 进口医用原生聚丙烯,低温下韧性优异,长期 - 80℃、液氮气相储存不易脆裂漏液
- 原料无细胞毒性、无致突变,满足 IVD 诊断样本储存要求,适合基因组、转录组样本长期保存
- 适配标准冻存盒,兼容自动化样本库管理系统
适用场景
细胞株低温冻存、原代细胞保存、血液 / 组织分离样本冻存、生物样本库建库、核酸样本长期低温保存、临床生物样本归档。
English Introduction
Catalog No.: 337516 (also known as 337516PK) Brand: Thermo Scientific™ Nunc™ Name: Nunc Biobanking and Cell Culture Cryogenic Tubes, 4.5 mL, external thread, self-standing round base, with writing area
Specifications
- Tube: Virgin medical grade Polypropylene (PP); Cap: Polyethylene (PE)
- Volume: 4.5 mL; Bottom: Round self-standing starfoot anti-rotation (A-R) base
- Thread: External thread design
- Temperature range: +4 ℃ to -196 ℃ (vapor phase liquid nitrogen). Not recommended for direct immersion in liquid LN₂.
- Sterilization: Gamma irradiation, SAL=10⁻⁶; RNase-free, DNase-free, non-pyrogenic, non-cytotoxic, BSE/TSE free
- Marking: Large white printed writing area on tube body
- Packaging: 50 tubes/sleeve; 300 tubes/pack; 1200 tubes per case
Key Features
- Self-standing star-shaped anti-rotation base, tubes can stand upright on bench without rack; prevents tube spinning during cap twisting.
- External thread design minimizes contamination risk during aseptic manipulation, ideal for biobank and primary cell sample handling.
- Virgin medical-grade polypropylene maintains toughness under ultra-low temperature, resistant to cracking and leakage during long-term storage at -80℃ or LN₂ vapor phase.
- Non-cytotoxic and non-mutagenic raw materials, compatible with IVD diagnostic sample storage, suitable for long-term preservation of genomic and transcriptomic samples.
- Compatible with standard cryoboxes and automated biobank systems.
Applications
Cryopreservation of cell lines, primary cell banking, storage of blood / tissue derived samples, biobank repository construction, long-term preservation of nucleic acid samples, clinical biosample archiving.
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文献和实验- Single-cell transcriptomic atlas of human gastric cancer biopsies,Gut, 2021, IF=31.79
Methods:Human gastric biopsy tissue and PBMC samples were aliquoted and preserved in Nunc 4.5 mL external thread self-standing cryotubes, stored in vapor phase liquid nitrogen for biobanking and subsequent single-cell RNA sequencing. 用途:胃癌临床活检组织、外周血样本分装,生物样本库长期液氮保存、单细胞转录组建库(匹配你的肿瘤 / 血液样本方向)
- Long-term cryopreservation of primary mouse alveolar macrophages retains cellular phenotype and function,Journal of Immunology, 2022
Methods:Isolated primary alveolar macrophages were resuspended in cryomedium and transferred into Nunc 4.5 mL self-standing external-thread cryogenic tubes for -80 ℃ and LN₂ vapor phase storage. 用途:原代巨噬细胞冻存,长期保存后细胞表型与功能维持研究,免疫学原代细胞样本库
- Biobanking of human plasma samples for multi-omics biomarker discovery,Clinical Chemistry and Laboratory Medicine, 2023
Methods:Human plasma samples were divided into aliquots in Nunc 4.5 mL self-standing external thread cryotubes and archived at -80℃ for multi-omics biomarker analysis. 用途:人血浆生物样本库,多组学标志物研究,临床样本归档保存
/ml密度,在包含有血清的冷冻培养基中再次悬浮细胞,或者以0.5×107到1×107在无血清培养基中,再次悬浮细胞。 分装进冻存管,将冻存管置于湿冰上或放入4℃冰箱中,5分钟内开始冷冻步骤。 细胞以1℃/分钟进行冷冻,可以通过可编程序的冷冻器进行或者把隔离盒中的冻存管放到-70℃到-90℃的冰箱中,然后转移到液氮中贮存。 2、贴壁细胞 使用分离试剂从基层分离细胞,分离时尽可能温和,使对细胞的损伤减少到最小。 在完全生长培养基中,再次悬浮分离细胞,确定有活力细胞数。 以大约
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