NUNC 156340 25cm细胞培养瓶,密封盖

NUNC（Thermo）156340 EasYFlask™ 25 cm² 细胞培养瓶（透气‑密封双位盖）中英文产品介绍

中文产品介绍

产品简介

Nunc EasYFlask™ T‑25 细胞培养瓶（货号156340），斜颈设计，双位透气 / 密封实心盖（Vent‑Close cap），Nunclon‑Delta TC 贴壁改性表面；高透明聚苯乙烯瓶身，蓝色 HDPE 螺旋盖；γ 射线辐照灭菌，无热原、无细胞毒性，无动物源成分。培养面积25 cm²，推荐工作体积 7 mL，总容积 70 mL；10 个 / 包，200 个 / 整箱。瓶盖带 Y 型可视标识，旋转 1/3 圈即可切换透气模式或完全密封模式，兼顾 CO₂培养箱通气培养、密闭转运 / 短期保存两种场景。

• 货号：156340
• 培养面积：25 cm²（T‑25）
• 瓶颈：斜颈（Canted‑neck）
• 瓶盖：双位透气‑密封实心盖（无滤膜），HDPE 蓝色；Y 标识可视化区分透气 / 密封档位
• 材质：瓶身 PS 聚苯乙烯；瓶盖 HDPE
• 推荐工作体积：7 mL；最大总容积 70 mL
• 表面处理：Nunclon‑Delta TC（贴壁细胞专用）
• 灭菌：γ 辐照灭菌 SAL 10⁻⁶
• 包装：10 个 / 小包；200 个 / 箱
• 质控：无热原、TSE/BSE‑free，多细胞株粘附验证

主要特点

1. 双模式瓶盖设计：仅旋转 1/3 圈，Y 标识位置肉眼判断状态；Y 朝上 = 透气，旋转到位完全拧紧实现密封；培养箱内通气培养，转运、低温静置时拧紧密封，不用更换瓶盖。
2. Nunclon‑Delta 亲水 TC 表面，适配绝大多数贴壁细胞、原代细胞，批次一致性高，细胞贴壁生长稳定。
3. 斜颈大口，移液管、细胞刮伸入操作方便；瓶身两侧模压 + 印刷双重体积刻度；侧壁微倾斜，倒置显微镜下可完整观察细胞状态；低矮瓶身，培养箱堆叠节省空间Thermo Fis...。
4. 高透明度原生聚苯乙烯，适合明场显微观察；γ 辐照无菌，无热原，无动物来源组分。
5. 同规格带滤膜透气盖版本货号：156367；本款为实心双位盖，无除菌滤膜。
6. 一次性耗材，禁止高压灭菌重复使用。

下游应用

贴壁细胞传代扩增；肿瘤细胞、原代细胞培养；药物干预细胞培养；细胞密闭转运、4 ℃短期样品存放；细胞学功能实验。

储存条件

室温干燥避光存放；一次性使用，禁止高压灭菌。

⚠️提示：透气档位仅适合 5% CO₂培养箱内；做密闭转运、4℃暂存务必完全拧紧至密封档位；不要用于长时间密闭培养。

English Product Introduction

Product Description

Nunc EasYFlask™ cell‑culture flask (Cat.No.156340), 25 cm² growth area, angled‑neck design, two‑position vent‑close solid screw cap. Nunclon‑Delta tissue‑culture‑treated surface for adherent cells. Clear virgin polystyrene body with blue HDPE cap. Gamma‑irradiated sterile, non‑pyrogenic, animal‑component‑free. Recommended working volume 7 mL, total capacity 70 mL. Pack size:10 flasks/pouch, 200 flasks per case. The cap features visual “Y” indicator; only 1/3‑turn switches between vented position for CO₂‑incubator culture and fully sealed position for transport or short‑term storage。

• Catalog No.: 156340
• Growth area: 25 cm² (T‑25)
• Neck type: Angled‑neck
• Cap: Two‑position vent‑close solid cap (no filter membrane), blue HDPE; “Y” mark for vent/seal visual confirmation
• Material: Flask body: polystyrene (PS); Cap: HDPE
• Recommended working volume:7 mL; total capacity:70 mL
• Surface: Nunclon‑Delta TC‑treated (adherent cells)
• Sterilization: Gamma‑irradiation SAL 10⁻⁶
• Packaging:10/pouch;200/case
• Quality: Non‑pyrogenic, TSE/BSE‑free, validated for multiple adherent cell lines

Key Features

1. Dual‑mode cap: Only one‑third turn to toggle status. Visible “Y” symbol indicates vented position; full tightening achieves complete sealing. One cap satisfies both incubator gas‑exchange and sample‑transport workflows.
2. Nunclon‑Delta hydrophilic TC surface ensures reliable attachment for adherent and primary cells with good batch‑to‑batch reproducibility.
3. Wide angled neck facilitates pipette and cell‑scraper access. Dual molded‑and‑printed graduations on both sides. Slightly angled side walls enable full microscopic observation. Low‑profile geometry optimizes incubator stacking space.
4. High‑clarity virgin polystyrene for bright‑field microscopy. Gamma‑sterile, non‑pyrogenic, animal‑free manufacturing.
5. Filter‑cap counterpart for constant ventilation: Cat.No.156367. This item uses solid two‑position cap without sterile filter membrane.
6. Single‑use consumable. Do not autoclave.

Applications

Adherent cell subculture and expansion; tumour‑cell & primary‑cell maintenance; drug‑treatment cell culture; cell sample transportation; short‑term 4 °C holding; cellular functional assays.

Storage

Store at room‑temperature in dry, dark environment. Single‑use only, autoclaving prohibited.

Note: Vented position is only intended for humidified 5 % CO₂ incubator. Tighten fully to sealed mode during transportation or cold temporary storage. Not for long‑term hermetic incubation.