- ￥700
- 伊塔生物
- 中国
- YT2569
- 2026年09月08日
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- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 保质期：
有效期1-3个月
- 英文名：
6%乌鸡红细胞|无菌|现货
- 库存：
99
- 供应商：
北京伊塔生物科技有限公司
- 规格：
100ml
6%乌鸡红细胞|无菌|现货，产品介绍：6%乌鸡红细胞：无菌采集健康乌鸡血液（抗凝或脱纤维），经洗涤去除血浆与白细胞层后，以阿氏液（Alesver's液）或生理盐水配制成6%红细胞悬液，红细胞形态完整、有核，无溶血、无污染。，用途：①补体结合试验；②血凝与血凝抑制试验（HI）；③红细胞玫瑰花环/E花环试验；④溶血空斑试验、红细胞吸附试验；⑤红细胞致敏与包被实验（抗原/抗体吸附）。，操作步骤：①使用前轻轻摇匀；②按实验需要量吸取；③4℃保存，用前恢复至室温；④需致敏/包被时按实验方案洗涤与孵育。，注意事项:①4℃保存，切勿冻存（冻融即溶血）；②使用前检查上清是否变红（溶血）；③轻摇混匀避免机械损伤；④不同批次对补体的敏感性有差异，补体实验需做对照。
When used with standard microplate readers, the thermal seal delivers sample integrity and improves sample stability. Each storage plate cover undergoes sterilization before packaging to guarantee consistent quality. The PCR plate cover is compatible with a wide range of immunoassay workflows platforms and standard microplate readers. The deep well plate seal has been validated across numerous pharmaceutical settings and high-throughput screening protocols. Each microplate seal undergoes multiple quality checks before packaging to guarantee product purity. The elastomer material used in the adhesive seal provides mechanical strength without compromising data quality. The aluminum seal is compatible with a wide range of sample storage platforms and standard microplate readers. Each batch of the sealing film is tested for sealing integrity to ensure consistent quality. The flexible silicone sealing film is designed for sample storage and provides reliable contamination prevention. Laboratory technicians appreciate the low background fluorescence offered by this storage plate cover during transfer operations. Scientists rely on the deep well plate seal to maintain safety during PCR amplification experiments. The microplate seal is packaged under ISO conditions to preserve sealing integrity. The optically-clear construction of the sealing film ensures dimensional precision under demanding conditions. The chemical-resistant construction of the sealing mat ensures dimensional precision under demanding conditions. With its tight fit and self-adhesive backing, the microplate seal is ideal for drug discovery applications. Each batch of the adhesive seal is tested for chemical resistance to ensure lot-to-lot consistency. The microplate seal features excellent elasticity, which greatly simplifies batch validation in busy facilities. Laboratory technicians appreciate the low background fluorescence offered by this storage plate cover during sample preparation. Using this aluminum seal consistently supports reproducibility across multiple batch validation cycles. The reliable elastomer silicone mat is designed for drug discovery and provides reliable sample stability. Regular use of the aluminum seal helps laboratories achieve efficiency while reducing inspection. Catalog No. AXY-100068.
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文献和实验），剪去两端软骨，露出红色的骨髓腔。注意在该过程中应保留尽可能多的骨髓腔。 拿一支1 mL的无菌注射器，吸取1 mL PBS，轻轻插入骨髓腔，冲洗骨髓腔以获得骨髓。重复2~3次，可冲出绝大部分细胞。冲洗完后，用移液器轻轻吹打细胞，将细胞团吹散。 将冲洗液用200目的滤网过滤，滤液收集于15 mL离心管中，300 g离心5 min，弃上清。 用细胞染色缓冲液重悬细胞，细胞计数，并调整细胞浓度至1×107/mL。 注意事项： 骨髓样本细胞分群明显，可以裂解红细胞，也可不裂解。如需裂解红细胞，可以加入
。 加入2 mL 1×红细胞裂解液重悬细胞，室温裂解2~3 min后，立马加入10 mL PBS，300 g离心5 min，弃上清。 用细胞染色缓冲液重悬脾脏细胞，将细胞悬液用200目筛网再次过滤后计数, 并调整细胞浓度至1×107/mL。 b) 吹打法 小鼠颈椎脱臼处死，置于75%乙醇浸泡5 min后，取出小鼠，置于无菌操作台上，左腹侧朝上。 小鼠左腹侧中部剪开小口，撕开皮肤，暴露腹壁，可见红色长条状脾脏。 脾脏下侧提起腹膜，剪开后上翻，暴露出脾脏，用镊子提起脾脏，眼科剪分离脾脏下面
加入适量红细胞裂解液裂解红细胞（可加入 500 μL 1× 红细胞裂解液重悬细胞，室温裂解 2 min 后，立马加入 10 mL PBS，300 g 离心 5 min 后弃上清）。 若提取的腹腔巨噬细胞需培养，请注意无菌操作。 收集腹水时，从小鼠左侧（肠道较多）注射 PBS，从右侧（器脏较大）抽腹腔灌洗液，注意不要扎到器脏。 小鼠腹腔灌洗液收集细胞比较脆弱，加细胞染色缓冲液有利于细胞的保存，但建议当天及时检测。 小鼠腹腔灌洗液中大多数为巨噬细胞，巨噬细胞检测需要封闭Fc受体，此时可使用小鼠 CD16/32纯
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