Anti-ATR抗体

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse

应用

ICC/IF, ChIP, WB, IP

applications

免疫原

Fusion protein corresponding to aa 400-500 of ATR, fused to ATR. The exact immunogen used to generate this antibody is proprietary information.

Q13535

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "

", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "

", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "

(in lysate from UV irradiated K562 cells).

", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "

" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "

", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "

", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "

" } } }

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

• Visit the General protocols
• Visit the Troubleshooting

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

Preservative: 0.05% Sodium azide Constituents: PBS

运输条件

Blue Ice

推荐的短期储存时间

1-2 weeks

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

靶点信息

Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor (PubMed : 10597277, PubMed : 10608806, PubMed : 10859164, PubMed : 11721054, PubMed : 12791985, PubMed : 12814551, PubMed : 14657349, PubMed : 14729973, PubMed : 14742437, PubMed : 15210935, PubMed : 15496423, PubMed : 16260606, PubMed : 21144835, PubMed : 21777809, PubMed : 23273981, PubMed : 25083873, PubMed : 27723717, PubMed : 27723720, PubMed : 30139873, PubMed : 33848395, PubMed : 37788673, PubMed : 37832547, PubMed : 9427750, PubMed : 9636169). Recognizes the substrate consensus sequence [ST]-Q (PubMed : 10597277, PubMed : 10608806, PubMed : 10859164, PubMed : 11721054, PubMed : 12791985, PubMed : 12814551, PubMed : 14657349, PubMed : 14729973, PubMed : 14742437, PubMed : 15210935, PubMed : 15496423, PubMed : 16260606, PubMed : 21144835, PubMed : 23273981, PubMed : 27723717, PubMed : 27723720, PubMed : 33848395, PubMed : 9427750, PubMed : 9636169). Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis (PubMed : 11114888, PubMed : 11418864, PubMed : 11865061, PubMed : 21777809, PubMed : 23273981, PubMed : 25083873, PubMed : 9925639). Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism (PubMed : 11673449). Required for FANCD2 ubiquitination (PubMed : 15314022). Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication (PubMed : 12526805). Acts as a regulator of the S-G2 transition by restricting the activity of CDK1 during S-phase to prevent premature entry into G2 (PubMed : 30139873). Acts as a regulator of the nuclear envelope integrity in response to DNA damage and stress (PubMed : 25083873, PubMed : 37788673, PubMed : 37832547). Acts as a mechanical stress sensor at the nuclear envelope : relocalizes to the nuclear envelope in response to mechanical stress and mediates a checkpoint via phosphorylation of CHEK1 (PubMed : 25083873). Also promotes nuclear envelope rupture in response to DNA damage by mediating phosphorylation of LMNA at 'Ser-282', leading to lamin disassembly (PubMed : 37832547). Involved in the inflammatory response to genome instability and double-stranded DNA breaks : acts by localizing to micronuclei arising from genome instability and catalyzing phosphorylation of LMNA at 'Ser-395', priming LMNA for subsequent phosphorylation by CDK1 and micronuclei envelope rupture (PubMed : 37788673). The rupture of micronuclear envelope triggers the cGAS-STING pathway thereby activating the type I interferon response and innate immunity (PubMed : 37788673). Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex (By similarity).

See full target information ATR