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- 技术资料
- 库存:
42
- 供应商:
广州市左克生物
- 规格:
100 μg
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产品简介:
货号:Z04982-100
产品名称:ABO hFc Chimera, Human
产品规格:100 μg
产品介绍文本:Histo-blood group ABH (O) antigens are major alloantigens in humans. These antigens are widely distributed in human tissues and undergo changes in expression during cellular differentiation and malignant development. The ABH antigens have been characterized as terminal disaccharide determinants w...
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| Species | Human | ||||
| Protein Construction |
|
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 60.56 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 62-70 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Supplied as 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 500mM L Arginine, 2mM DTT (pH 7.0). | ||||
| Concentration | Verified by one or more methods from A280/Bioactivity/BCA/Bradford. | ||||
| Storage & Stability | This product remains stable for 6 months at -80°C or below. Avoid repeated freeze-thaw cycles. |
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技术背景HTML:
| Target Background | Histo-blood group ABH (O) antigens are major alloantigens in humans. These antigens are widely distributed in human tissues and undergo changes in expression during cellular differentiation and malignant development. The ABH antigens have been characterized as terminal disaccharide determinants which represent secondary gene products. They are synthesized in a stepwise fashion from a precursor by the action of different glycosyltransferases. |
| Synonyms | ABH |
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文献和实验in this way are, by definition, specific for the targeted animal and might not bind to the human homologues. For this reason we have developed a novel approach using in vivo phage display selection against human tissues transplanted into SCID animals
ABO(H) Blood Group Expression on Circulating Glycoproteins
ABO(H) blood group antigens are typical carbohydrate antigens widely expressed on erythrocytes, digestive tissue, respiratory tissue, and in secreted body fluids such as milk, urine, and saliva. These antigens
cells. We present here (i) a detailed protocol for the production and purification of soluble recombinant NK cell receptors tagged with human IgG1-Fc (thus termed receptor-Fc chimera or receptor-Ig fusion protein) and (ii) a protocol for cell staining
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