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- 文献和实验
- 技术资料
- 抗体名:
PE-Cy7标记的CD3抗体
- 抗体英文名:
Anti-CD3(PE-Cy7) Monoclonal Antibody
- 靶点:
CD3
- 浓度:
FCM 5uL/Test.
- 应用范围:
FCM
- 宿主:
Rat
- 供应商:
北京索莱宝科技有限公司
- 库存:
999
- 级别:
实验级
- 目录编号:
12500/12502/12501/12503
- 保质期:
Store at 2-8℃ in dark. Do not freeze.
- 标记物:
Biotin,Cy3,Cy3.5,Cy5,Cy5.5,Cy7,FITC,HRP,RBITC,SAlexa Fluor 350,SAlexa Fluor 405,SAlexa Fluor 488,SAlexa Fluor 532,SAlexa Fluor 555,SAlexa Fluor 568,SAlexa Fluor 594,SAlexa Fluor 633,SAlexa Fluor 640,SAlexa Fluor 647,SAlexa Fluor 660,SAlexa Fluor 680,SAlexa Fluor 750
- 克隆性:
Monoclonal Antibody
- 保存条件:
Store at 2-8℃ in dark. Do not freeze.
- 形态:
干粉/液体
- 亚型:
IgG2b, κ
- 规格:
100T/50T
| 规格: | 100T | 产品价格: | ¥900.00 |
|---|---|---|---|
| 规格: | 50T | 产品价格: | ¥500.00 |
【抗体靶点简介】
The protein encoded by this gene is the CD3-epsilon polypeptide,which together with CD3-gamma,-delta and -zeta,and the T-cell receptor alpha/beta and gamma/delta heterodimers,forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon,gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.【实验操作视频参考】
![]() Western blot实验参考操作流程 | ![]() Immunohistochemistry实验参考操作流程 |
抗体在科研领域应用十分广泛,对解析生物学过程、探究疾病发病机制、创新药物研发与临床治疗研究均起到关键支撑作用。
索莱宝现有30000余种经过严格实验验证的抗体产品,全面覆盖神经科学、细胞凋亡、表观遗传学等热门研究方向,适配 WB、IHC、ELISA、IP、co-IP 等主流实验技术场景。包括内参标签抗体,以及多种标记类型的一抗、二抗,充分满足客户多样化实验选型需求。索莱宝拥有成熟专业的抗体制备与质量检测平台,从免疫制备、纯化提纯到实验验证全流程严格筛选评估,全方位保障每一支抗体的性能与品质。
此外,索莱宝已完善一站式技术服务平台,可提供 WB、IHC、IF、IP、流式检测、细胞实验等常规检测,以及多克隆/单克隆抗体定制等个性化定制服务,为科研用户提供全方位解决方案。
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文献和实验Conjugation of monoclonal antibodies
derivatives, and Texas Red, may not work well for some IgM antibodies. Nonetheless, it is probably worth a try--we have had stunning success conjugating IgM antibodies with PE, APC, and Cy7APC. The conjugation of immunoglobulins is very straight-forward
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
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