Rabbit PDK1 Antibody 抗体,orb1527164,Biorbyt产品图

Rabbit PDK1 Antibody 抗体,orb152

7164,Biorbyt
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  • ¥3159 - 7839
  • Biorbyt已认证
  • orb1527164
  • 英国
  • 2026年08月11日
  • IP, WB
  • Rabbit
  • Human, Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Rabbit PDK1 Antibody 抗体

    • 抗体英文名

      Rabbit PDK1 Antibody

    • 靶点

      PDK1

    • 浓度

      1000 µg/ml

    • 应用范围

      IP, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb1527164

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      详询

    • 规格

      10 ul/100 ul

    规格:10 ul产品价格:¥3159.0
    规格:100 ul产品价格:¥7839.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: PDK1 Antibody

    别名: 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; hPDK1; PDK1; PDPK2; PDPK2P; PkB kinase like gene 1; PRO0461

    应用稀释比例: WB - 1:2,000 - 1:10,000; IP - 5 - 10 µg/mg lysate

    应用说明: Format: Whole IgG

    免疫原: Between 506 and C-terminus

    防腐剂: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

    纯化方法: Antigen Affinity Purified

    研究领域: Signal Transduction

    UniProt ID: O15530

    NCBI 参考序列: NP_002604.1

    保存条件: 2 - 8°C

    备注: For research use only.

    产品细节图片1
    Detection of human and mouse PDK1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50µg) cells. Antibodies: Affinity purified rabbit anti-PDK1 antibody orb1527164 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

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    图标文献和实验
    相关实验
    • Generation and Selection of Rabbit Antibody Libraries by Phage Display

      The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs

    • Generation of Rabbit Immune Libraries

      gene that is preferentially used, and the diversity of the antibody repertoire being mainly generated by gene conversion and hypermutation, which facilitates the molecular cloning of the rabbit immune repertoire. In this chapter, the general strategy

    • Testing Rabbit Bleeds By Elisa

      at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody

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