- ￥8775
- Biorbyt
- orb2635135
- 英国
- 2026年08月07日
- IHC-P, WB
- Rabbit
- Human
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- 文献和实验
- 技术资料
- 抗体名：
Recombinant HSP27 Antibody / HSPB1 抗体
- 抗体英文名：
Recombinant HSP27 Antibody / HSPB1
- 应用范围：
IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb2635135
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Recombinant
- 保存条件：
详询
- 亚型：
Rabbit IgG, kappa
- 免疫原：
详询
- 规格：
100 ug
产品子类型: Primary Antibody
描述: HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
克隆号: HSPB1/7038R
应用稀释比例: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
应用说明: Optimal dilution of therecombinant HSP27 antibody should be determined by the researcher.
免疫原: Recombinant human full-length protein was used as the immunogen for the recombinant HSP27 antibody.
防腐剂: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
纯化方法: Protein A/G affinity
研究领域: Neuroscience
UniProt ID: P04792
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
allow unique approaches for interrogating global protein interaction networks. Protein arrays can be divided into two categories: antibody arrays and functional protein arrays. Antibody arrays consist of various antibodies and are appropriate
DIRECT ELISA USING FLUORESCENT SUBSTRATE PROTOCOL
Day 1:1. Coat with 100 μl/well of coating antibody diluted in filtered PBS. Incubate plate overnight at 4°C, coveredwith plate sealer.Day 2:2. Block plates with 200 μl/well of 4 g Block ACE powder, diluted in 100 ml of deionized water (USE a 1
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