
Plant Retinoblastoma related p
rotein Antibody 植物抗体(Anti-RBR1)相关产品推荐更多 >
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- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型:
Rabbit IgG
- 形态:
Lyophilized
- 保存条件:
-20°C.
- 克隆性:
Polyclonal
- 标记物:
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期:
long term storage (1 year or more) at -20°C.
- 抗原来源:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别:
科研
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度:
Concentration after reconstitution: 1 µg/µL
- 抗体英文名:
Retinoblastoma related protein
- 抗体名:
Anti-RBR1
- 规格:
100 µl
Anti-RBR1 | Retinoblastoma related protein
AS11 1627| Clonality:Polyclonal| Host:Chicken| Reactivity:Arabiodpis thaliana, Medicago sativa
Product Info
| Immunogen: | Recombinant C-terminal fragment consisting of 236 amino acids of Arabidopsis thaliana retinoblastoma protein UniProt: Q9LKZ3, lTAIR: At3g12280 |
| Host: | Chicken |
| Clonality: | Polyclonal |
| Purity: | Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide. |
| Format: | Liquid |
| Quantity: | 100 µl |
| Storage: | Store at 4°C. Upon arrival Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | Chromatin Immunoprecipitation (ChIP), ChIP-qPCR, Immunoprecipitation (IP), Western blot (WB) |
| Recommended dilution: | 1.5 µl (ChIP-qPCR), 2 µl (IP), 1 : 2000 (WB) |
| Expected | apparent MW: | 112 kDa |
Reactivity
| Confirmed reactivity: | Arabidopsis thaliana, Medicago sativa |
| Predicted reactivity: | Camelia sinensis, Chenopodium rubrum, Cocos nucifera, Hordeum vulgare, Oryza sativa, Pisum sativum, Populus tremula, Scutelaria baicalensis, Zea mays Species of your interest not listed? Contact us |
| Not reactive in: | Chlamydomonas reinhardtii |
Additional Information
Additional information (application): This antibody is not suitable for immunolocalization. Methanol concentration in a transfer buffer can be considerably reduced or for a better transfer of high MW proteins (even with PVDF membrane). For immunoprecipitation start with 2 µl and titrate it depending upon your experimental conditions. Please note that you work with a total IgY fraction, which means that it will contain between 40-60 µg of total IgY (directed not only against retinoblastoma) therefore all of this IgY needs to be captured by the anti-IgY matrix. As control pre-serum for IP this product can be used, total, pre-immune IgY .
Background
The retinoblastoma protein Rb is considered to be a key regulator of G1/S phase transition by blocking S phase entry and cell growth. Plant retinoblastoma-related (RBR) proteins share a homology with the human tumour suppressor retinoblastoma (pRb) protein. RBR protein functions are controlled by phosphorylation and protein-protein interactions. Short name: AtRBR
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