- ￥4329 - 10647
- Biorbyt
- orb1946115
- 英国
- 2026年08月07日
- FC, WB
- Rabbit
- Human
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- 文献和实验
- 技术资料
- 抗体名：
Phospho-MEK1/2 (Ser217/221) rabbit mAb Antibody 抗体
- 抗体英文名：
Phospho-MEK1/2 (Ser217/221) rabbit mAb Antibody
- 浓度：
0.5 mg/mL
- 应用范围：
FC, WB
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1946115
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 形态：
Liquid
- 亚型：
Rabbit IgG1k
- 免疫原：
详询
- 规格：
20 ul/200 ul
| 规格： | 20 ul | 产品价格： | ￥4329.0 |
|---|---|---|---|
| 规格： | 200 ul | 产品价格： | ￥10647.0 |
描述: Mitogen-activated protein kinase (MAPK) is the main building block of the intracellular signaling network. The signals are initiated by activation of a small G protein (e.g., Ras), followed by a sequential activation of several sets of protein kinases. The extracellular signal-regulated kinase (ERK) pathway is stimulated by a large number of extracellular stimuli as well as various internal processes. This cascade regulates processes related to proliferation, differentiation, development, and oncogenic transformation. The signaling is usually initially started by activation of small G proteins (e.g. Ras), which in turn recruits to the membrane and activates the MAP3K (Raf kinase). The cascade leads to the activation of MAP kinase kinases (MEKs). MEKs are phosphorylated at Ser218 and Ser222 in MEK1. The phosphorylation activates MEKs. Phosphorylation of MEK at other sites regulates its activity as well. For example, phosphorylation at Ser386 by ERKs may either inhibit ERKs activity or under certain condition, facilitate the activation of ERKs by enhancing the MEK1 binding to Grb10 scaffold protein. Phosphorylation of MEK1 at Ser298 by p21-activating protein (PAK1) may lead to its activation whereas this can be inhibited by a feedback phosphorylation of MEK1 at Thr292 by ERKs. Protein Ser/Thr phosphatase inactivates MEK by dephosphorylation of pSer218 and pSer222. Other phosphatases my also regulate MEK activity. Upon activation, MEKs behaves as a dual kinase and phosphorylate key Tyr and Thr residues of ERKs, thus causing their activation.
克隆号: BJ4
别名: Dual specificity mitogen-activated protein kinase kinase 1/2, MAPK/ERK kinase 1, MAPKK1, MAPKK2, MAP2K1, MAP2K2, PRKMK1, PRKMK2
应用稀释比例: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
免疫原: A synthetic phospho-peptide corresponding to residues surrounding Ser217/221 of human phospho MEK1/2.
交叉反应: Predicted to work with mouse, rat and other homologues.
防腐剂: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
纯化方法: Protein A+G
研究领域: Signal Transduction
UniProt ID: P36507, Q02750
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
antibody unconjugated； 货号21337，产品描述：GAPDH Antibody；规格 50 ul / 100 ul； 货号3052，产品描述：Rabbit anti-Human IgG secondary antibody HRP conjugated； 货号300904050，产品描述：One-Hour Western kit，10 ml / bottle x 3；
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号；因此，我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂，我们则建议使用与二抗来源相同的血清进行封闭。抗体：Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本：石蜡
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