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- 文献和实验
- 技术资料
- 抗体名:
VAV1 (Phospho-Tyr174) Antibody 抗体
- 抗体英文名:
VAV1 (Phospho-Tyr174) Antibody
- 浓度:
1mg/ml
- 应用范围:
WB
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb223132
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
详询
- 免疫原:
详询
- 规格:
25 ug/50 ug/100 ug/200 ug
| 规格: | 25 ug | 产品价格: | ¥1404.0 |
|---|---|---|---|
| 规格: | 50 ug | 产品价格: | ¥2106.0 |
| 规格: | 100 ug | 产品价格: | ¥3393.0 |
| 规格: | 200 ug | 产品价格: | ¥4797.0 |
描述: VAV1 (Phospho-Tyr174) Antibody is an unconjugated polyclonal antibody. It is suitable for use in WB. It exhibits reactivity with Human, Mouse, Rat.
别名: anti VAV1 (Phospho-Tyr174) antibody
应用稀释比例: WB: 1:500-1:3000, ELISA: 1:10000
免疫原: Internal
研究领域: Signal Transduction
UniProt ID: P15498
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.

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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
。所以,我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体:Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本:石蜡包埋人类肺部肿瘤样本煮沸设备:浴锅 (左),微波炉 (中),高压锅 (右)免疫染色—封闭在 IHC–P 中,我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品,时长 1 小时,以防止非特异性背景染色。在进行 IHC–F 时,在含有 0.3% Triton. X–100 的 1X
Making antibodies to synthetic peptides
You'll need to couple the synthetic peptide hapten to a protein carrier, with a bifunctional reagent. For antibody development, we don't bother to use highly purified peptide. Crude (unpurified) peptide or partially purified peptide
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