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- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
Biorbyt
- 检测范围:
93.75-6000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
34.26 pg/mL
- 规格:
48 T/96 T
| 规格: | 48 T | 产品价格: | ¥3861.0 |
|---|---|---|---|
| 规格: | 96 T | 产品价格: | ¥5499.0 |
描述: Rat EPO(Erythropoietin) Microsample ELISA Kit
别名: EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
应用说明: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EPO in the samples is then determined by comparing the OD of the samples to the standard curve.
检测时间: 3.5h
研究领域: Hematology, Immunology & Inflammation, Metabolism Research
UniProt ID: P29676
备注: For research use only.

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文献和实验【申请上传】Current Protocols in Immunology
Immunocompromised Animals Unit 1.3 Handling and Restraint Unit 1.4 Anesthesia Unit 1.5 Animal Identification Section II Use of Mouse, Rat, Hamster, and Rabbit Unit 1.6 Parenteral Injections Unit 1.7 Blood Collection Unit 1.8 Euthanasia Section III Selected
Size‐Exclusion Chromatography with On‐Line Light Scattering
. Chem. 269:27840‐27846. Philo, J.S., Aoki, K.H., Arakawa, T., Narhi, L.O., and Wen, J. 1996a. Dimerization of the extracellular domain of the erythropoietin (EPO) receptor
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