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- 文献和实验
- 技术资料
- 保存条件：
2-8度
- 保质期：
1年
- 英文名：
CST3 Active
- 库存：
999
- 供应商：
上海淳麦生物
- 规格：
/5ug/20ug/1mg
Recombinant Human Cystatin C Active
英文简称:
CST3 Active
中文名称:
重组人半胱氨酸蛋白酶抑制剂C，活性
背景资料:
Cystatins are a superfamily of cysteine proteinase inhibitors found in both plants and animals. They comprise a group of proteinase inhibitors， widely distributed in tissues and body fluids， and form tight complexes with cysteine proteases such as cathepsin B， H， L and S. Cystatin C， a secreted molecule of this family， is of interest from biochemical， medicine and evolutionary points of view. Cystatin C， with molecular weight of 13260 Da， is composed of 120 amino acids， lacks carbohydrate and has two disulfide bridges located near the carboxyl terminus. Cystatin C is increased in patients with malignant diseases， and is related to the insu人胚肾HEK细胞重组表达iciency of renal function and appears to be a better marker than creatinine. On the other hand， low levels of cystatin C involve cause the breakdown of the elastic laminae and， subsequently， the atherosclerosis and abdominal aortic aneurysm.
产品描述:
Cystatin-C Human Recombinant produced in HEK cells is a non-glycosylated monomer， having a molecular weight of approximately 13kDa. The Cystatin-C is purified by proprietary chromatographic techniques.
来源宿主:
人胚肾HEK细胞重组表达
溶解建议:
提供的重组人半胱氨酸蛋白酶抑制剂C，活性产品形式为冻干粉末.建议用无菌处理的18M-cm水溶解重组人半胱氨酸蛋白酶抑制剂C，活性，浓度不低于100ug/ml.后继实验过程中可根据实验要求进一步稀释.
保存建议：
重组人半胱氨酸蛋白酶抑制剂C，活性产品在室温保存3周，于-18°C以下长期保存.一旦溶解，于4°C保存2-7天.长期存储建议添加0.1%HSA或BSA载体蛋白.避免反复冻融
应用领域：
重组人半胱氨酸蛋白酶抑制剂C，活性产品可广泛应用于酶活性研究、代谢研究、功能研究、蛋白互作研究、ELISA检测、Western Blot、免疫组化等生物医学研究领域。
关键词：
重组人半胱氨酸蛋白酶抑制剂C，活性、Recombinant Human Cystatin C Active、CST3 Active、重组蛋白、酶类、哺乳细胞表达、CMBIO、淳麦生物
其他简介：
CMBIO
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文献和实验Quantification of Active Caspase-3 and Active Caspase-8 in Microglia Cells
During microglia activation the levels of active caspase-3, caspase-7 and caspase-8 are increased, which leads to the transcription of proinflammatory cytokines and factors. As such, the induction of caspase activity in microglia can be used
Immunoblotting for Active Caspase-1
Immunoblotting for caspase-1 is the gold-standard method of detecting inflammasome activation. In contrast to IL-1β-based readouts, it can be used in an experimental setup independent of de novo gene expression. Here, we present protocols
that need to be optimized, including the choice of antibody, the conditions for shearing chromatin using sonication and the analysis of immunoprecipitated DNA. The ChIP-IT Kit from Active Motif provides a complete solution to all potential problems associated with the ChIP
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