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- 文献和实验
- 技术资料
- 保存条件:
2-8度
- 保质期:
1年
- 英文名:
TXN1, His
- 库存:
999
- 供应商:
上海淳麦生物
- 规格:
/5ug/20ug/1mg
Recombinant E.Coli Thioredoxin, His
英文简称:
TXN1, His
中文名称:
重组大肠杆菌硫氧还蛋白,His标签
背景资料:
Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have bRecombinant Thioredoxin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids (2-109 a.a.) and having a molecular mass of 12.9kDa. TRX contains an 11 amino acid His Tag N-terminus and is purified by proprietary chromatographic techniques.n found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. Trx also provides control over a number of transcription factors a大肠杆菌中重组表达ecting cell proliferation and death through a mechanism referred to as redox regulation. It has bRecombinant Thioredoxin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids (2-109 a.a.) and having a molecular mass of 12.9kDa. TRX contains an 11 amino acid His Tag N-terminus and is purified by proprietary chromatographic techniques.n suested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an e大肠杆菌中重组表达icient oxidoreductant. This could be especially useful in
产品描述:
Recombinant Thioredoxin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids (2-109 a.a.) and having a molecular mass of 12.9kDa. TRX contains an 11 amino acid His Tag N-terminus and is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达
溶解建议:
Sterile Filtered colorless solution. The TRX His Tag protein solution contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1mM DTT.
保存建议:
TRX His Tag although stable 4℃ for 4 weeks, should be stored desiccated below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
应用领域:
重组大肠杆菌硫氧还蛋白,His标签产品可广泛应用于功能研究、蛋白互作研究、ELISA检测、Western Blot、免疫组化等生物医学研究领域。
关键词:
重组大肠杆菌硫氧还蛋白,His标签、Recombinant E.Coli Thioredoxin, His、TXN1, His、重组蛋白、多肽、原核表达、His标签、CMBIO
其他简介:
CMBIO




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文献和实验硫氧还蛋白还原酶 thioredoxin reductase
由还原型 NADP催化硫氧还蛋白还原的酶。 EC1. 6. 4. 5.。含有一个分子的 FAD。使氧化型硫氧还蛋白的胱氨酸残基还原,变成一对半胱氨酸残基,后者进一步成为核糖核苷酸还原的电子供体。它存在于大肠杆菌、酵母、肝脏以及肿瘤细胞中。
人 硫氧还蛋白还原酶( TrxR ) 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中 硫氧还蛋白还原酶(TrxR ) 的含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人 硫氧还蛋白还原酶( TrxR ) 水平。用纯化的人 硫氧还蛋白还原酶( TrxR ) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 硫氧还蛋白还原酶( TrxR
人硫氧还蛋白相互作用蛋白(TXNIP)酶联免疫分析(ELISA)
人硫氧还蛋白相互作用蛋白(TXNIP ) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中硫氧还蛋白相互作用蛋白(TXNIP )的 含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中 人硫氧还蛋白相互作用蛋白 (TXNIP ) 水平。用纯化的 人硫氧还蛋白相互作用蛋白 (TXNIP )抗体 包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入
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