重组人p53，GST标签(p53 GST)

产品英文名称：
Recombinant Human p53 GST-Tag

英文简称:
p53 GST

中文名称:
重组人p53，GST标签

背景资料:
Tumor protein p53 responds to various cellular stresses by regulating target genes that induce cell cycle arrest， apoptosis， senescence， DNA repair， or changes in metabolism. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth， replication， and apoptosis. p53 is a DNA-binding protein containing transcription activation， DNA-binding & oligomerization domains. p53 binds to mdm2， SV40 T antigen and human papilloma virus E6 protein p53 senses DNA damage and possibly facilitating repair. p53 protein is a transcription factor which is encoded in humans by the TP53 gene. Alterations of TP53 occur not only as somatic mutations in human malignancies， but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. p53 mutants that often occur in many di大肠杆菌中重组表达erent human cancers fail to bind the consensus DNA binding site， and hence cause the loss of tumor suppressor activity. Mutation involving p53 is found in a wide variety of malignant tumors， including breast， ovarian， bladder， colon， lung， and melanoma. The p53 expression in normal cells is low and in an assortment of transformed cell lines is high， which may contribute to transformation and malignancy. Multiple p53 variants encode distinct isoforms， which can regulate p53 transcriptional activity. p53’s significance in multicellular organisms is in cell cycle regulation therefore it functions as a tumor suppressor that is involved in preventing cancer. p53’s role in conserving stability by preventing genome mutation has earned it descriptions such as "the guardian of the genome，" "the guardian angel gene，" and the "master watchman.” The name p53 refers to its evident molecular mass: it migrates as a 53kDa protein on SDS-PAGE. However， based on calculations from its amino acid residues， p53’s mass is in fact only 43.7kDa. This di大肠杆菌中重组表达erence is attributed to the high number of proline residues in the protein which slow its migration on SDS-PAGE， consequently making it appear larger than it actually is.

产品描述:
p53 Human Recombinant full length produced in E.Coli is a non-glycosylated， polypeptide chain having a total Mw of 81kDa. p53 Human Recombinant is fused to GST tag and purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达

溶解建议:
Sterile Filtered clear solution. Purified human p53 in 50mM Tris-Acetate， pH-7.5， 1mM EDTA and 20% glycerol.

保存建议：
For long term storage store at -20℃. Avoid freeze/thaw cycles.
应用领域：
重组人p53，GST标签产品可广泛应用于免疫检测研究、抗体开发、功能研究、蛋白互作研究、ELISA检测、Western Blot、免疫组化等生物医学研究领域。

关键词：
重组人p53，GST标签、Recombinant Human p53 GST-Tag、p53 GST、重组蛋白、多肽、抗原、原核表达、标签蛋白

其他简介：

 
CMBIO