- ￥1268
- abinscience
- France
- HY039022
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in 20 mM Tris-HCl pH 8.0, 100 mM NaCl,1mM EDTA, 4%trehalose,2377% mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Human TP53/p53 Protein, N-GST
- 库存：
999
- 供应商：
abinscience
- 规格：
50ug
| Product name | Recombinant Human TP53/p53 Protein, N-GST |
|---|---|
| Catalog No. | HY039022 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | P04637 |
| Protein length | Glu2-Asp393 |
| Nature | Recombinant |
| Predicted molecular weight | 70.34 kDa |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative Names | Antigen NY-CO-13, Cellular tumor antigen p53, P53, Tumor suppressor p53, TP53, Phosphoprotein p53 |
| Species | Homo sapiens (Human) |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验以最火热的 p53 为例Step1 先找 p53 基因序列1. NCBI--输入 p53 并选择物种 human：tumor protein p53 [Homo sapiens (human)]https://www.ncbi.nlm.nih.gov/gene2. Ctrl+F，输入 sequence，快速定位（当然其实拉下去就是了），点击 Genebank3. 下载 p53 基因序列4. 用 SnapGene 软件打开序列，并简单了解每个元件最好是可以学一下 SnapGene 这个软件的使用
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
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