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- 文献和实验
- 技术资料
- 抗体名:
NR5A2/LRH1 Antibody 抗体
- 抗体英文名:
NR5A2/LRH1 Antibody
- 靶点:
NR5A2 / LRH1
- 浓度:
500 ug/mL
- 应用范围:
ELISA, WB
- 宿主:
Goat
- 适应物种:
Human, Porcine
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb1248278
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
详询
- 形态:
Liquid
- 免疫原:
详询
- 规格:
0.1 mg
产品子类型: Primary Antibody
描述: NR5A2/LRH1 Antibody
别名: NR5A2, nuclear receptor subfamily 5, group A, member 2, B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2, CYP7A promoter-binding factor, alpha-1-fetoprotein transcription factor, b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus, fetoprotein-alpha 1 (AFP) transcription factor, hepatocytic transcription factor, liver nuclear receptor homolog-1 variant 2, liver receptor homolog 1, liver receptor homolog-1, nuclear receptor NR5A2, nuclear receptor subfamily 5 group A member 2
应用说明: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in nuclear lysates of cell lines HeLa and HepG2, and approx 60kDa band observed in Pig Ovary lysates (calculated MW of 56.5kDa according to Human NP_003813.1 and Pig AFD98842.1). Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver and NIH-3T3 cell line for this particular batch.
免疫原: The immunogen for this antibody is: C-TDYDRSPFVTSPIS
分子量: Approx 55kDa
预测反应性: Bovine, Canine, Mouse, Rat
防腐剂: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
纯化方法: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
特异性: This antibody is expected to recognize both reported isoforms (NP_995582.1; NP_003813.1).
研究领域: Stem Cell & Developmental Biology
UniProt ID: O60587
NCBI 参考序列: NP_995582.1, NP_003813.1
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.


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文献和实验a subset of genomic DNA sequences of type 5'-(NNN )3 -3', whereas the 6ZF library would recognize a subset of genomic sequences of type 5'-(NNN )6 -3'. Given the zinc-finger domains used, both libraries were more likely to recognize (RNN)x -type sequences
Phage‐Based Expression Cloning to Identify Interacting Proteins
the interacting protein. This technique leads directly to the isolation of a cDNA encoding the interacting protein, bypassing the need for labor?intensive protein purification, microsequencing, or antibody production.
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
Homo sapiens matrix metalloproteinase-like 1 (MMPL1), mRNA 695 NM_001950 Homo sapiens E2F transcription factor 4, p107/p130-binding (E2F4), mRNA 956 NM_003815 Homo sapiens a disintegrin and metalloproteinase domain 15 (metargidin) (ADAM15), mRNA
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