- ￥1960
- BOSTER
- PB0326-100ul
- 中国
- 2025年07月08日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | PB0326 |
|---|---|
| 产品名称 | Anti-NR5A2 Antibody |
| 基因名 | NR5A2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 61KD |
| 免疫原 | E.coli-derived human NR5A2 recombinant protein (Position: K44-R237). Human NR5A2 shares 95% amino acid (aa) sequence identity with both mouse and rat NR5A2. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | NR5A2 (nuclear receptor subfamily 5, group A, member 2) also known as liver receptor homolog-1 (LRH-1) is a protein that in humans is encoded by the NR5A2 gene. LRH-1 is a member of the nuclear receptor family of intracellulartranscription factors. LRH-1 plays a critical role in the regulation of development,cholesterol transport, bile acid homeostasis andsteroidogenesis. LRH-1 is important for maintaining pluripotence of stem cells during embryonic development. Liver receptor homolog-1 has been shown to interact with the small heterodimer partner. |
| 研究类别 | 1. Goodwin, B., Jones, S. A., Price, R. R., Watson, M. A., McKee, D. D., Moore, L. B., Galardi, C., Wilson, J. G., Lewis, M. C., Roth, M. E., Maloney, P. R., Willson, T. M., Kliewer, S. A. A regulatory cascade of the nuclear receptors FXR, SHP-1, and LRH-1 represses bile acid biosynthesis. Molec. Cell 6: 517-526, 2000.2. Gu, P.; Goodwin, B.; Chung, A. C. -K.; Xu, X.; Wheeler, D. A.; Price, R. R.; Galardi, C.; Peng, L.; Latour, A. M.; Koller, B. H.; Gossen, J.; Kliewer, S. A.; Cooney, A. J. (2005). "Orphan Nuclear Receptor LRH-1 is Required to Maintain Oct4 Expression at the Epiblast Stage of Embryonic Development". Molecular and Cellular Biology 25(9): 3492–3505. |
| Uniprot ID | NR5A2: O00482 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大
[list_product_images]Figure 1. Western blot analysis of NR5A2 using anti-NR5A2 antibody (PB0326). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: PANC Whole Cell Lysate,Lane 2: HEPG2 Whole Cell Lysate,Lane 3: A549 Whole Cell Lysate,Lane 4: SMMC Whole Cell Lysate,Lane 5: HELA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR5A2 antigen affinity purified polyclonal antibody (Catalog # PB0326) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR5A2 at approximately 61KD. The expected band size for NR5A2 is at 61KD.[/list_product_images]
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
Anti-NR5A2/LRH1 Antibody(PB0326-100ul)
￥1960
