- ￥3042 - 4563
- Biorbyt
- orb1294239
- 英国
- 2026年07月31日
- IF, IHC, WB
- Mouse
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
AKT Mouse Monoclonal Antibody 抗体
- 抗体英文名：
AKT Mouse Monoclonal Antibody
- 靶点：
AKT
- 应用范围：
IF, IHC, WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1294239
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgG1
- 免疫原：
详询
- 规格：
25 ul/100 ul
| 规格： | 25 ul | 产品价格： | ￥3042.0 |
|---|---|---|---|
| 规格： | 100 ul | 产品价格： | ￥4563.0 |
产品子类型: Primary Antibody
描述: Mouse Monoclonal antibody to AKT
应用稀释比例: Western Blot 1:500-1:1000, Immunofluorescence 1:100-1:200, Immunohistochemistry (Paraffin) 1:50-1:100
防腐剂: Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
研究领域: Signal Transduction
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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