- ￥6446
- OriGene
- TA325176
- 美国
- 2026年04月01日
- WB
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Mouse monoclonal AKT1 Antibody(Ascites)
- 抗体英文名：
Mouse monoclonal AKT1 Antibody(Ascites)
- 靶点：
AKT1
- 应用范围：
WB
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
TA325176
- 级别：
科研级
- 库存：
2周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store at -20°C as received.
- 形态：
Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
- 免疫原：
This AKT1 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the 430-460 region of human AKT1.
- 规格：
100 ul
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文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
In Vivo and In Vitro Production of Monoclonal Antibodies: Bioreactors vs Immune Ascites
. The first has been the introduction of contaminating proteins. The second has been variability in the capacity of hybridomas to elicit ascites and produce antibody. The third has been the constraint of needing to use syngeneic mice or nude mice as host animals for the production
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