- ￥5499
- Biorbyt
- orb623891
- 英国
- 2026年07月26日
- ELISA, FC, IHC, KO/KD Validated, WB
- Rabbit
- Human, Mouse
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- 文献和实验
- 技术资料
- 抗体名：
COX2/Cyclooxygenase 2/PTGS2 Rabbit Polyclonal Antibody 抗体
- 抗体英文名：
COX2/Cyclooxygenase 2/PTGS2 Rabbit Polyclonal Antibody
- 靶点：
Prostaglandin G/H synthase 2
- 浓度：
500 µg/ml
- 应用范围：
ELISA, FC, IHC, KO/KD Validated, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb623891
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Lyophilized
- 亚型：
Rabbit IgG
- 免疫原：
详询
- 规格：
100 ug
类别: Antibodies
产品子类型: Primary Antibody
描述: Anti-COX2/Cyclooxygenase 2/PTGS2 Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse.
别名: COX 2; COX2; COX2/ Cyclooxygenase 2; COX2/ Cyclooxygenase 2/ PTGS2; Cyclooxygenase 2; GRIPGHS; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PHS 2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; PTGS2
应用稀释比例: Western blot, 0.1-0.25μg/ml, Human, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human ELISA, 0.1-0.5μg/ml
免疫原: E.coli-derived human COX2/Cyclooxygenase 2/PTGS2 recombinant protein (Position: A18-L604).
交叉反应: No cross-reactivity with other proteins.
分子量: 75 kDa
防腐剂: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg NaN3.
纯化方法: Immunogen affinity purified.
复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
特异性: No cross reactivity with other proteins.
研究领域: Cancer Biology, Cancer Biology; Metabolism Research, Cardiovascular Research, Hematology, Metabolism Research, Signal Transduction
UniProt ID: P35354
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: Anti-COX2/Cyclooxygenase 2/PTGS2 Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse.
别名: COX 2; COX2; COX2/ Cyclooxygenase 2; COX2/ Cyclooxygenase 2/ PTGS2; Cyclooxygenase 2; GRIPGHS; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PHS 2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; PTGS2
应用稀释比例: Western blot, 0.1-0.25μg/ml, Human, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human ELISA, 0.1-0.5μg/ml
免疫原: E.coli-derived human COX2/Cyclooxygenase 2/PTGS2 recombinant protein (Position: A18-L604).
交叉反应: No cross-reactivity with other proteins.
分子量: 75 kDa
防腐剂: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg NaN3.
纯化方法: Immunogen affinity purified.
复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
特异性: No cross reactivity with other proteins.
研究领域: Cancer Biology, Cancer Biology; Metabolism Research, Cardiovascular Research, Hematology, Metabolism Research, Signal Transduction
UniProt ID: P35354
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
Flow Cytometry analysis of CACO-2 cells using anti-PTGS2 antibody. Overlay histogram showing CACO-2 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% Fixative and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGS2 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of HEPA1-6 cells using anti-PTGS2 antibody. Overlay histogram showing HEPA1-6 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% Fixative and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGS2 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
IHC analysis of PTGS2 using anti-PTGS2 antibody. PTGS2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-PTGS2 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of PTGS2 using anti-PTGS2 antibody. PTGS2 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-PTGS2 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Western blot analysis of PTGS2 using anti-PTGS2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse RAW264.7(-LPS) whole cell lysates, Lane 3: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS2 antigen affinity purified polyclonal antibody at 0.25 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTGS2 at approximately 75 kDa. The expected band size for PTGS2 is at 69 kDa.
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COX2/Cyclooxygenase 2/PTGS2 Rabbit Polyclonal Antibody 抗体，orb623891，Biorbyt
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