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文献和实验Standard Protocols Autoradiography (35S)
). The developer should be a 1:1 dilution in water. The fixer is 30% (w/v) of sodium thisulphate. Place slides in a dark box and carry to dark room. Also take a slide rack, tweezers and the emulsion. Switch on the red light and switch
NMWL 30 000 cartridges (at 6000 rpm in microcentrifuge), wash through with 2x400ul water, collect +/-20ul sample: this is pure enough for sequencing. NOTE: Product is clean enough for restriction digest immediately after reaction; however, phenol
Stewart Laboratory Standard Operating Procedure
BrdU Incorporation Protocol Day 1 Label cells with media containing100 μM BrdU (6 μL/mL of 5 mg/mL stock): Diploid Fibroblasts, 4-6 hours ES cells, 30 minutes 3T3 cells, 2.5 hours Trypsinize cells, wash from plate with PBS, spin down (400
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