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RT. Do not freeze.
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5g///50g
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文献和实验by placing the gel slice within a microfuge tube containing a membrane with a small pore size, such as a Costar Spin-X microfuge tube fitted with a 0.22 μm filter [12]. Alternatively, gel slices composed of low melting point (LMP) agarose can be placed
Synthesis of Peptidomimetic Conjugates of Cyclic Nucleoside Phosphonates
, 80 Å pore size, 21.2 × 150–mm; Beckman Coulter) Silica gel thin‐layer chromatography (TLC) plates (Merck) Preparative
with a salt solution (300 mM NaCl) of high ionic strength. (C ) Ca2+ induces defects on a supported membrane containing 25% POPA. Defects appear upon addition of 0.5 mM CaCl2 and gradually increase in size with time. (D ) and (E ) Examples of diffusion
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