AAV Gi-GPCR Assay Kit-A3AR

The AAV Gi-GPCR Assay Kit-A3AR is designed specifically for screening drugs/compounds that target the Adenosine A3 Receptor (A3AR) by combination AAV.
The Adenosine A3 Receptor (A3AR) is a type of G protein-coupled receptor (GPCR) that binds to adenosine, an endogenous purine nucleoside. A3AR is primarily coupled with the Gi/Go class of G proteins, which, upon activation, inhibit adenylate cyclase, leading to a decrease in cyclic AMP (cAMP) levels. Additionally, A3AR can influence other signaling pathways, including the activation of phospholipase C (PLC) and the regulation of intracellular calcium levels. A3AR is expressed in various tissues, including the heart, liver, brain, and immune cells. It is involved in several physiological and pathological processes, such as inflammation, ischemic protection, and cancer progression. Due to its diverse roles, A3AR is a potential therapeutic target for treating inflammatory diseases, cancer, and cardiovascular conditions. The receptor's activation can have both protective and detrimental effects, depending on the context and tissue type involved.
This kit uses AAV vectors with a CMV promoter to co-express the A3AR and cyclic nucleotide-gated (CNG) channel, allowing researchers to conduct high-throughput screening and functional analysis of potential A3AR-targeting compounds. The kit provides a sensitive and reliable method for evaluating the pharmacological properties of A3AR drugs, such as agonists and antagonists, in a live-cell environment.