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- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Human IgG1 - kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Humanized
- 应用范围:
ELISA; Flow Cyt
- 浓度:
详见COA
- 靶点:
CD19
- 抗体英文名:
Anti-CD19 (inebilizumab biosimilar)mAb
- 抗体名:
Anti-CD19 (inebilizumab biosimilar)mAb
- 规格:
50μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | CD19 |
| Uniprot ID | P15391 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| 通用名 | MEDI-551,Medi-551 |
| 货期 | In Stock |
| 产品英文名称 | Anti-CD19 (inebilizumab biosimilar)mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | 12 months from date of dispatch |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 别名 | CD19;4;VID3;GC12802 |
| 应用 | ELISA; Flow Cyt |
| 有无偶联 | Unconjugated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 宿主种属 | Humanized |
| IgG亚型 | Human IgG1 - kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000; Flow Cyt 1:100 |
| 应用范围 | Research use only |
Figure 1. Flow cytometry analysis with Anti-CD19 mAb 1 μg/ml (inebilizumab) on HEK293 cells transfected with human CD19 (Blue histogram) or HEK293 transfected with irrelevant protein(Red histogram).
Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD19 Protein, mFc Tag (PME100781) can bind Anti-CD19 (inebilizumab biosimilar) mAb (BME100051) in a linear range of 0.64–16 ng/mL.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验CD19 分子 CD19 常用单克隆抗体或代号: B4,Leu12 主要表达细胞: B,Pre,FDC [B] 分子质量(kDa)和结构: gp90(IgSF) 功 能: 与CD21、CD81组成复合物,调节B细胞活化 CD19 Response regulator that plays a dominant role in establishing signaling
CD19 / CD81 白细胞分化抗原及其功能 CDl9 CDl9分子胞膜外区结构属IgSF,包括2个C2样区,胞质区较长,不同种属间有很高的同源性,含有可发生磷酸化的丝/苏氨酸和酪氨酸残基。CDl9分子分布于除浆细胞外的B细胞谱系发育的各个阶段,也是与B细胞识别、黏附和活化有关的CD分子的结构B细胞的重要标记。 此外CDl9还表达于生发中心的FDC,以及某些急性髓细胞白血病细胞。CDl9是CDl9/CD21/CD81信号复合
抗G4S linker抗体:一文讲清"通用连接肽"检测的原理与选型
抗G4S linker抗体的核心优势 在抗linker抗体普及之前,CAR阳性率检测多采用靶抗原-Fc融合蛋白染色:比如检测CD19 CAR,使用CD19-Fc结合scFv,再通过荧光二抗读取信号。该方法在单靶点场景可用,但存在明显局限,而抗G4S linker抗体可以很好弥补这些不足: 通用型检测,降低试剂开发成本 传统抗原-Fc试剂靶点专一,更换CAR靶点就需要重新表达纯化蛋白,工作量大;抗G4S linker抗体识别VH、VL之间的连接肽序列,只要构建体携带(G4S)n重复,无论靶点是CD19
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