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- 规格:
100 mL
BI Serum-free Cell Freezing Medium(无血清细胞冻存液)
产品货号(型号):05-065-1B
产品规格:100 mL
核心优势:
- ✅ 无动物源成分,不含血清,避免批次差异与外源污染风险;
- ✅ 预混型即用配方,含优化浓度的DMSO、渗透保护剂及细胞膜稳定剂;
- ✅ 经多种哺乳动物细胞(如HEK293、CHO、Jurkat、iPSC等)验证,复苏存活率≥90%;
- ✅ 无需现配现用,减少操作误差,提升冻存标准化水平;
- ✅ 符合ISO 13485质量管理体系生产,无菌过滤(0.22 μm),内毒素<0.1 EU/mL。
同系列产品:
- 05-065-1A — Serum-free Cell Freezing Medium,50 mL
- 05-065-1C — Serum-free Cell Freezing Medium,500 mL
- 05-066-1A — CryoStor® CS10(BI合作授权版,含10% DMSO),50 mL
- 05-067-1B — Serum-containing Cell Freezing Medium(含10% FBS),100 mL
- 05-068-1A — DMSO-Free Cell Freezing Medium(无DMSO配方),50 mL
FAQ:
- Q:是否需要预热或平衡至室温使用?
A:建议于4℃冷藏保存,使用前平衡至室温(15–25℃),避免冷激损伤细胞。 - Q:可否用于原代细胞或干细胞冻存?
A:已成功应用于人源MSC、iPSC及原代神经元等敏感细胞,推荐冻存密度为0.5–2×10⁶ cells/mL。 - Q:复苏后是否需立即离心去除冻存液?
A:建议复苏后轻柔离心(200–300 × g, 5 min),弃上清并换用新鲜培养基,以降低DMSO残留影响。 - Q:能否长期-80℃暂存后再转入液氮?
A:可于-80℃短期存放(≤1周),但为保障最佳活力,建议程序降温后直接转入液氮气相长期保存。 - Q:该产品含酚红或抗生素吗?
A:不含酚红、不含抗生素;成分透明,便于后续表型/功能检测——具体组分请以COA及产品标签为准。
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