Recombinant SARS-CoV-2 Spike S

Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets

., Janjua, H., Shastry, R., Ho, C.K., Wang, D., Wang, H., Jiang, M., Montelione, G.T., Stuart, D.I., Owens, R.J., Daenke, S., Schutz, A., Heinemann, U., Yokoyama, S., Bussow, K., and Gunsalus, K.C. 2008. Protein production and purification. Nat. Methods

Cell Death Differ：中大五院黄曦团队发现新冠病毒引起肺损伤的分子机制

上皮细胞线粒体凋亡，并导致肺水肿的发生。 图片来源：Cell death & differentiation 病毒相关蛋白与细胞蛋白相互作用影响细胞凋亡过程。作者首先分别将携带刺突蛋白（Spike protein, S 蛋白），核衣壳蛋白（Nucleocapsid, N 蛋白），膜蛋白（Membrane protein, M 蛋白）和包膜蛋白（Envelope protein，E 蛋白）真核表达质粒载体分别转染人肺上皮 H292 细胞和人血管内皮 EA.hy926 细胞，通过荧光分光光度法检测胞内活化

Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions

., Simmons, L., Singh, J., Smith, V., Stinson, J., Vagts, A., Vandlen, R., Wantanabe, C., Wiend, D., Woods, K., Xie, M.H., Yansura, D., Yi, S., Yu, G., Yuan, J., Zhang, M., Zhang, Z., Gaddard, A., Wood, W.I., Godowski, P., and Gray, A. 2003. The secreted