- ￥254 - 7864
- TargetMol
- T6984
- 2026年07月07日
2 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- CAS号：
709002-46-0
- 规格：
1mg/1mLx10mM(inDMSO)/5mg/10mg/25mg/50mg/100mg/500mg
| 规格： | 1mg | 产品价格： | ￥254.0 |
|---|---|---|---|
| 规格： | 1mLx10mM(inDMSO) | 产品价格： | ￥622.0 |
| 规格： | 5mg | 产品价格： | ￥550.0 |
| 规格： | 10mg | 产品价格： | ￥789.0 |
| 规格： | 25mg | 产品价格： | ￥1584.0 |
| 规格： | 50mg | 产品价格： | ￥2632.0 |
| 规格： | 100mg | 产品价格： | ￥3808.0 |
| 规格： | 500mg | 产品价格： | ￥7864.0 |
Product Introduction
Bioactivity
| 名称 | SirReal2 |
| 描述 | SirReal2 is a potent and selective Sirt2 inhibitor with IC50 of 140 nM. |
| 细胞实验 | HeLa cells were plated in petri dishes (5?cm), incubated overnight to a confluency of 30–40% and then treated with SirReal2 dissolved in RPMI1640 medium supplemented with fresh 20% (v/v) FCS, 1% (v/v) penicillin, 1% (v/v) streptomycin, 1% (v/v), L-glutamine, 1% (v/v) DMSO for 5?h at various concentrations. Cells were then washed with prewarmed PBS (2?ml), lysed in SDS–PAGE sample buffer (70?μl, 50?mM Tris/HCl, 0.5?mM EDTA, 1 × Complete Protease Inhibitors, 2% (v/v) IGEPAL, 2% (w/v) SDS, 10% (v/v) glycerol, 50?mM NCA, 3.3?μM trichostatin A, 50?mM DTT, 0.01% (w/v) bromophenol blue, pH 6.8) and sonicated (5?min). Cell samples were then separated using SDS–PAGE (12.5% (w/v) polyacrylamide), transferred to an activated nitrocellulose membrane (Bio-Rad), blocked with non-fat dry milk (Roth, 5% (w/v), TBS, 0.1% (v/v) Tween 20) and probed with an anti-acetyl-α-tubulin antibody (1:1,000) and an anti-GAPDH antibody (1:2,000–1:5,000) as a loading control. |
| 激酶实验 | Wee1 Mass Screening: Wee1 mass screening is performed using Amersham's p34cdc2 kinase SPA kit with some modifications. Briefly, 45–60 nM full-length Wee1 kinase is incubated with 25 μM compounds, 20 μM ATP, and 122–441 nM Cdc2/cyclin B in a final volume of 50 μl of enzyme dilution buffer [50 mM Tris (pH 8.0), 10 mM NaCl, 10 mM MgCl2, 1 mM DTT, and 0.1 mM Na3VO4]. After 30 min incubation at 30°C, 30 μl of [33P]ATP containing kinase buffer [67 mM Tris (pH 8.0), 40 mM NaCl, 13 mM MgCl2, 1 mM DTT, and 0.13 mM Na3VO4] containing 1 μM biotinylated peptide, and 0.25 μCi of [γ-33P]ATP is added to the reaction and incubated for another 30 min at 30°C. The reaction is stopped by adding 200 μl of stop buffer [50 μM ATP, 5 mM EDTA, 0.1% Triton X-100, and 1.25 mg/ml SPA beads in PBS]. After centrifugation at 2400 rpm for 15 min, the plate is counted with Wallac's Microbeta counter. |
| 体外活性 | SirReal2 selectively inhibits Sirt2 via a Sirt2-specific amino acid network, and induces a tubulin hyperacetylation and a significant depletion of BubR1 in HeLa cells. [1] |
| 体内活性 | In vivo, SirReal2 inhibits Sirt2 activity without affecting the activity of the other Class-I sirtuins Sirt1 and Sirt3. [1] |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | 10% DMSO+90% Saline : < 6.25 mg/mL (14.86 mM), Lower concentrations may be soluble, but exact solubility limit is unknown. 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 6.25 mg/mL (14.86 mM), Solution. DMSO : 62.5 mg/mL (148.61 mM), Sonication is recommended. |
| 关键字 | Sirtuin | SIRT2 | SirReal-2 | SirReal2 | SirReal 2 | Inhibitor | inhibit |
| 相关产品 | Selisistat | Fisetin | Nicotinamide riboside | Nicotinamide | Dihydrocoumarin | MDL-800 | Nicotinamide riboside malate | Sirtinol | JGB1741 | SRT 2104 | SIRT5 inhibitor 8 | MC3482 |
| 相关库 | Inhibitor Library | Bioactive Compound Library | Anti-Cancer Compound Library | Bioactive Compounds Library Max | Histone Modification Compound Library | Highly Selective Inhibitor Library | Anti-Aging Compound Library | NO PAINS Compound Library | Antioxidant Compound Library | DNA Damage & Repair Compound Library | Microtubule-Targeted Compound Library | Reprogramming Compound Library |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
官网有文献
相关实验
网络 第十九章 杂环化合物和生物碱 杂环化合物在自然界分布很广，其数量几乎占已知有机化合物的三分之一，用途也很多。许多重要的物质如叶绿素、血红素、核酸以及临床应用的一些有显著疗效的天然药物和合成药物等，都含有杂环化合物的结构。生物碱多是中草药的有效成分，绝大多数是含氮的杂环化合物。本章内容与医学关系密切，具有重要意义。
分子中具有高能键的化合物。在生物体内主要有上式（Ⅰ）和（Ⅱ）两种结构的高能化合物。其作用为贮藏能量（例如 ATP、肌酸磷酸）和作为产生 ATP源的中间代谢产物（例如磷酸烯醇丙酮酸）以及合成反应的中间物质（例如酰基辅酶 A），在蛋白质的结构（ conformation）变化周期的第一阶段上（例如钠钾 ATP酶的羧基磷酸中间体）也具有重要作用。多数高等化合物具有磷酸基，但也有例外。
由不同种元素组成的纯净物叫做化合物。化合物一般有固定的组成。化合物的组成一般可用化学式表示。化合物具有确定的物理性质和化学性质，不同于其组成元素的性质。化合物中的元素不能用简单的机械方法或物理方法分开，而必须用化学方法才能分离。
技术资料暂无技术资料 索取技术资料
文献支持
化合物 SirReal2【709002-46-0】
￥254 - 7864
