- ￥254 - 740
- TargetMol
- T1272
- 2026年07月07日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- CAS号：
147-94-4
- 规格：
1mLx10mM(inDMSO)/1g/50mg/100mg/500mg
| 规格： | 1mLx10mM(inDMSO) | 产品价格： | ￥368.0 |
|---|---|---|---|
| 规格： | 1g | 产品价格： | ￥740.0 |
| 规格： | 50mg | 产品价格： | ￥254.0 |
| 规格： | 100mg | 产品价格： | ￥334.0 |
| 规格： | 500mg | 产品价格： | ￥617.0 |
Product Introduction
Bioactivity
| 名称 | Cytarabine |
| 描述 | Cytarabine (Ara-C) is a nucleoside analog, an inhibitor of DNA synthesis (IC50=16 nM). Cytarabine inhibits DNA polymerase and induces cell cycle arrest, autophagy, and apoptosis. Cytarabine has antitumor activity. |
| 细胞实验 | Cells are incubated in the presence of different concentrations of Cytarabine at 37 °C for 24, 48, and 72 hours. At the time of 20-, 44-, or 68-hour incubation in the presence of Cytarabine, 10 mL of cell proliferation reagent WST-1 solution is added. After 2- and 4-hour incubation with WST-1, cell metabolic activity is assessed with colorimetric changes quantified by measuring the absorbance in a spectrophotometer at 450 nm. And cell division times are calculated from eosin counting in parallel with viability assay(Only for Reference) |
| 激酶实验 | In Vitro Growth Inhibition Assay: Stock solution of Cytarabine is prepared in absolute ethanol, and serial dilutions of Cytarabine are prepared. CCRF-CEM cells are suspended in RPMI medium supplemented with 10% FBS, 0.1% gentamicin, and 1% sodium pyruvate. The cells are suspended in their respective media to give 10 mL volumes of cell suspension at a final density of 3-6 × 104 cells/mL. Appropriate volumes of Cytarabine solution are transferred to the cell suspensions, and incubation is continued for 72 hours. The cells are spun down and resuspended in fresh Cytarabine -free medium, and final cell counts are determined. The data are analyzed by sigmoidal curve fitting of the cell count versus Cytarabine concentration, and the results are expressed as the IC50 (Cytarabine concentration that inhibits cell growth to 50% of the control value). |
| 体外活性 | 方法：原代 AML 细胞用 Cytarabine (10-5000 nM) 处理 24 h，使用 MTT 方法检测细胞活力。 结果：Cytarabine 具有剂量依赖性的生长抑制作用，IC50 为1.12 μM。[1] 方法：人组织细胞淋巴瘤细胞 U937 用 Cytarabine (10-1000 nM) 处理 72 h，使用 Flow Cytometry 检测细胞周期情况。 结果：Cytarabine 剂量依赖性地降低了用 10 和 100 nM 处理的细胞中 G1 期的细胞比例，并增加了 S 期的比例。当以最高剂量 1000 nM 施用时，Cytarabine 引起亚 G1 期和 G2/M 期比例的显著增加。[2] |
| 体内活性 | 方法：为研究 AML 的化疗耐药性，将 Cytarabine (10-60 mg/kg) 腹腔注射给携带人 AML 肿瘤的 NSG 小鼠，每天一次，持续五天。 结果：在所有接受 60 mg/kg Cytarabine 治疗的小鼠中，在治疗后 2 周出现细胞还原作用，治疗后 4-13 周复发。最低白血病负担和外周血复发时间方面，模型中存在剂量-反应关系。[3] 方法：为检测体内抗肿瘤活性，将 Cytarabine (6.25 mg/kg，每天一次) 和 sorafenib (60 mg/kg，每天两次) 腹腔注射给携带人 AML 肿瘤 U937 的 NOD-SCID-IL2R γnull 小鼠模型，每周一次或持续四十八天。 结果：Cytarabine 与 sorafenib 联合使用在体内产生了强大的抗 AML 活性。[4] |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 5 mg/mL (20.56 mM), Sonication is recommended. DMSO : 262.5 mg/mL (1079.27 mM), Sonication is recommended. H2O : 24.3 mg/mL (99.91 mM), Sonication is recommended. |
| 关键字 | RNASynthesis | RNA Synthesis | Orthopoxvirus | NucleosideAntimetabolite | Nucleoside Antimetabolite/Analog | Nucleoside Antimetabolite | Inhibitor | inhibit | HSV | Herpes simplex virus | EndogenousMetabolite | Endogenous Metabolite | DNASynthesis | DNA/RNA Synthesis | DNA Synthesis | Cytosine beta-D-arabinofuranoside | Cytosine b-D-arabinofuranoside | Cytarabine | Autophagy | Apoptosis | Analog |
| 相关产品 | Guanidine hydrochloride | Glycerol | Aceglutamide | Malic acid | DL-Lysine | Sucrose | Hemin | Nicotinamide riboside malate | Gluconate Calcium | Thymidine | Corn starch | D(+)-Raffinose pentahydrate |
| 相关库 | Anti-Cancer Active Compound Library | Bioactive Compound Library | Bioactive Compounds Library Max | Failed Clinical Trials Compound Library | Anti-Aging Compound Library | Anti-Viral Compound Library | Traditional Chinese Medicine Monomer Library | Natural Product Library | Anti-virus Traditional Chinese Medicine Monomer Library | Natural Product Library for HTS | Anti-infective Natural Product Library | Anti-Cancer Drug Library |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验适应症的临床应用，仅作为辅助支持治疗，已有大量文献报道，本文不再赘述。由于rhG-CSF同时具有免疫调节作用，已有医学专家开始尝试应用rhG-CSF作为治疗方案用药，并取得了某些疗效。目前主要应用的领域是治疗急性髓性白血病的CAG预激方案。 近年来，急性髓性白血病的M3亚型，也即急性早幼粒细胞白血病（APL）的治疗有了重大突破。上海血液学研究所应用全反式维甲酸和三氧化二砷制剂联合用药，使APL患者达到94.1%的完全缓解率，五年无事件生存率和总体生存率分别达到89.2%和91
94-114 AAACAGCTGTTGTTGAGCGAA IMR90E1A hCdc2 (24) 125-147
常用化学试剂分子量 Molecular Weights of Commonly Used Chemicals
94.11 Ammonium Chloride 53.49 Phenylmethylsulfonyl fluoride (PMSF) 174.2 Ammonium Persulfate 228.2 Polyethylene glycol c. 8000
技术资料暂无技术资料 索取技术资料
