相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
从原料到标记,打造属于中国自己的流式抗体品牌Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 检测范围:
0.156-10 ng/mL
- 应用:
Sandwich ELISA
- 适应物种:
Mouse
- 样本:
Tissue lysate
- 规格:
96T
Product Information
KE10224 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-15RA ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-15RA. The assay recognizes mouse IL-15RA. An antibody specific for mouse IL-15RA has been pre-coated onto the microwells. The mouse IL-15RA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse IL-15RA is added to detect the captured mouse IL-15RA protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-15RA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate 备注:对未验证样本,我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16169 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 101% | 86%-122% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 6.65 | 0.42 | 6.28 |
| 2 | 8 | 1.32 | 0.05 | 3.53 |
| 3 | 8 | 0.71 | 0.01 | 1.68 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 7.35 | 0.89 | 12.11 |
| 2 | 16 | 1.34 | 0.05 | 3.99 |
| 3 | 16 | 0.71 | 0.05 | 7.29 |
Background Information
Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2. These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively. The IL-15Rα chain is expressed by many cell types, including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD215, IL-15 receptor subunit alpha, Il15ra, IL-15R-alpha, Interleukin-15 receptor subunit alpha |
FAQ
1、我的样本稀释液有限,如何处理稀释倍数较大的样本?
我们建议您对样本进行梯度稀释。例如,人血清样本需要1000000倍稀释时,您可以分3个梯度,每个梯度100倍,进行稀释:
第一步:取2 uL人血清样本,加入198 uL样本稀释液,混匀得到100倍稀释的人血清样本
第二步:取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释,混匀得到10000倍稀释的人血清样本
第三步:取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释,混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释,您可以使用更多梯度。除了我们推荐的样本稀释液,不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足,请联系我们的客服为您提供更多样本稀释液。
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验大小不一样的引物对。3) 将RNA提取物用RNase-free的DNase I 处理。本试剂盒还可以用于DNase I处理后的RNA清洁(cleanup),请联系我们索取具体操作说明书。4) 在步骤去蛋白液RW1漂洗前,直接在吸附柱RA上进行DNase I处理。请联系我们索取具体操作说明书。6. RNA 纯度及浓度检测:完整性: RNA 可用普通琼脂糖凝胶电泳 (电泳条件:胶浓度1.2%;0.5×TBE电泳缓冲液;150v,15 分钟)检测完整性。由于细胞中70%-80%的RNA 为rRNA,电泳后UV
特异性。需要用不同的抗原和酶联物来检测相应的抗体,不可相互“通用” 。因此试剂生产成本较高。 其他应用: · ELISA:采用双抗夹心ELISA检测HSV1。 · 免疫胶体金:利用免疫层析原理制备免疫胶体金试纸条来检测HSV1。 · 免疫荧光:采用自制荧光标记HSV1 McAb以直接免疫荧光法(DFA)检测HSV1,以期可获得一种用于临床应用的快速检测HSV抗原的方法。 八、四正柏生物简介 北京四正柏生物科技有限公司(Beijing
检测下限仅4.78 pg/mL!Elabscience未包被人白介素3(IL-3) elisa试剂盒,让细胞因子检测更高效
内容概要 Elabscience未包被人白介素3(IL-3)elisa试剂盒专为具备丰富经验的科研用户设计,提供包括捕获抗体、检测抗体在内的核心组分,具有高灵敏度(4.78 pg/mL)、宽检测范围(31.25-2000 pg/mL)及高特异性。采用经典的双抗夹心法原理,适用于血清、血浆及细胞培养上清等多种样本类型,是进行免疫学、血液学及炎症研究的理想工具。 产品介绍 产品名称:未包被人白介素3(IL-3)酶联免疫吸附测定试剂盒 英文名称:Uncoated Human IL
技术资料暂无技术资料 索取技术资料







