重组Influenza A H3N2 HA trimer蛋白(Y114F, His & AVI 标签), 生物素标记,41056-V49H1-B

重组Influenza A H3N2 HA trimer蛋白(Y114F, His & AVI 标签), 生物素标记（产品说明）
蛋白名称：重组Influenza A H3N2 HA trimer蛋白(Y114F, His & AVI 标签), 生物素标记
蛋白构建：A DNA sequence encoding the Influenza A H3N2 (A/District of Columbia/27/2023) Hemagglutinin / HA trimer (translated amino acids of EPI2990337), termed as HA, was expressed with the bacteriophage T4 fibritin and a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
蛋白活性：1.Immobilized Recombinant Influenza A H3N2 HA trimer Protein (Y114F, His & AVI Tag), Biotinylated(Cat: 41056-V49H1-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-Influenza HA (Research Grade Clone CR8020), the EC50 is 1-4 ng/mL(QC tested). 2.Immobilized Recombinant Influenza A H3N2 HA trimer Protein (Y114F, His & AVI Tag), Biotinylated(Cat: 41056-V49H1-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-Influenza HA (Research Grade Clone CR9114), the EC50 is 2.2 ng/mL (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gln 17
蛋白分子量：The recombinant Influenza A H3N2 (A/District of Columbia/27/2023) Hemagglutinin / HA trimer comprises 573 amino acids and has a predicted molecular mass of 64.2 kDa. It migrates as an approximately 82.6 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 245.7 kDa verified by SEC-MALS.
蛋白NP号：EPI2990337
蛋白氨基酸序列：Met1-Val521