ZymoMag Protein A ^ (200 ul)

DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)

. Discard the supernatant. Air dry the protein pellet by inverting the tube on a absorbent paper for 5-10 minutes. 29. Add 100-200uL protein loading dye (Laemmli Loading dye) to the tube to dissolve the protein pellet. If the protein

SQ Blood Mini Protocol for 50ul Clotted Blood

lysate and invert 10 time to mix throughly. Incubate the tube at 37°C for 5 minutes. 7. Place the tube on ice for 1 minute. Add 200 ul PCP buffer to the cell lysate. Vortex vigorously at high speed for 30 seconds to mix. Some protein clumps

RNA Purification Protocol for 100-200 mg of Animal Tissue

设备   1. Centrifuge capable of 3,000-5,000 x g 2. High speed centrifuge tube with capacity of at least 15 ml 3. Ice bath 4. Ground pestle 实验步骤   1. Dissect tissue quickly and freeze in liquid nitrogen