- ￥350 - 1200
- GenScript
- A01849
- 2026年06月29日
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- 规格：
250 U (5U/μl)/1000 U (5U/μl)
| 规格： | 250 U (5U/μl) | 产品价格： | ￥350.0 |
|---|---|---|---|
| 规格： | 1000 U (5U/μl) | 产品价格： | ￥1200.0 |
| Specificity | Taq DNA polymerase |
| Host Species | Mouse |
| Immunogen | Taq DNA polymerase |
| Concentration | 5 U/μl |
| Storage Instructions | The product remains stable for 12 months at -20°C from date of receipt. Avoid repeated freezing and thawing cycles. |
The primer extension assay to detection the blocking activity of Hot Start Taq Antibody. All groups were added with 1.25 U Taq DNA polymerase pretreated with 0, 0.25, 0.5, 1, 2 U (Lane 1-5) Hot Start Taq Antibody.»
The PCR specificity detection of Hot Start Taq Antibody. The Antibody used in each group is 2, 1, 0.5, 0 U (lane 1-2, 3-4, 5-6, 7-8).»
| Target Background | The Hot Start Taq Antibody is a mouse monoclonal antibody that specifically binds to Taq DNA polymerase. When this antibody is bound to Taq, the enzyme is rendered inactive. It provides an antibody-mediated hot start that enhances the specificity and sensitivity of PCR. Inhibition is completely reversed when the temperature is raised during the first denaturing step of thermal cycling. |
| Synonyms | Taq Antibody |
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文献和实验Hot Start activation approaches are increasingly being used to improve the performance of PCR. Since the inception of Hot Start as a means of blocking DNA polymerase extension at lower temperatures, a number of approaches have been developed
Antibody to an equal amount of Taq DNA polymerase units. ForTaq DNA polymerase at 5 unit per μl, prepare a 1:1 mixture. Mix well using a Vortex Mixer. 3. Add the following components to a sterile 0.5-ml microcentrifuge tube:
for both DNA sample and reaction mixture preparation, is strongly recommended.The reagents for PCR should be prepared separately and used solely for this purpose. Autoclaving of all solutions, except dNTPs, primers and Taq DNA Polymerase is recommended
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